Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept.
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Date
2021-02-17
Open Access Location
Authors
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Journal ISSN
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Publisher
Frontiers Media S.A.
Rights
CC BY 4.0
(c) 2021 The Author/s
(c) 2021 The Author/s
Abstract
Isolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10-21 day) "cold enrichment" protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with culture on a non-selective medium, and subsequent identification of suspect colonies using elastic light scatter (ELS) analysis. A prototype database of ELS profiles from five Yersinia species and six other bacterial genera found in pork mince was established, and used to compare similar profiles of colonies obtained from enrichment cultures from pork mince samples seeded with representative strains of Y. enterocolitica and Y. pseudotuberculosis. The presumptive identification by ELS using computerised or visual analyses of 83/90 colonies in these experiments as the target species was confirmed by partial 16S rDNA sequencing. In addition to seeded cultures, our method recovered two naturally occurring Yersinia strains. Our results indicate that modified enrichment combined with ELS is a promising new approach for expedited detection of foodborne pathogenic yersiniae.
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Keywords
Yersinia, detection, diagnostics, elastic light scatter, food
Citation
On SLW, Zhang Y, Gehring A, Patsekin V, Chelikani V, Flint S, Wang H, Billington C, Fletcher GC, Lindsay J, Robinson JP. (2021). Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept.. Front Microbiol. 12. (pp. 641801-).