Genomic adaptations of Campylobacter jejuni to long-term human colonization

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dc.citation.issue1
dc.citation.volume13
dc.contributor.authorBloomfield SJ
dc.contributor.authorMidwinter AC
dc.contributor.authorBiggs PJ
dc.contributor.authorFrench NP
dc.contributor.authorMarshall JC
dc.contributor.authorHayman DTS
dc.contributor.authorCarter PE
dc.contributor.authorMather AE
dc.contributor.authorFayaz A
dc.contributor.authorThornley C
dc.contributor.authorKelly DJ
dc.contributor.authorBenschop J
dc.coverage.spatialEngland
dc.date.accessioned2024-01-11T20:08:33Z
dc.date.accessioned2024-07-25T06:39:33Z
dc.date.available2021-12-10
dc.date.available2024-01-11T20:08:33Z
dc.date.available2024-07-25T06:39:33Z
dc.date.issued2021-12-10
dc.description.abstractBACKGROUND: Campylobacter is a genus of bacteria that has been isolated from the gastrointestinal tract of humans and animals, and the environments they inhabit around the world. Campylobacter adapt to new environments by changes in their gene content and expression, but little is known about how they adapt to long-term human colonization. In this study, the genomes of 31 isolates from a New Zealand patient and 22 isolates from a United Kingdom patient belonging to Campylobacter jejuni sequence type 45 (ST45) were compared with 209 ST45 genomes from other sources to identify the mechanisms by which Campylobacter adapts to long-term human colonization. In addition, the New Zealand patient had their microbiota investigated using 16S rRNA metabarcoding, and their level of inflammation and immunosuppression analyzed using biochemical tests, to determine how Campylobacter adapts to a changing gastrointestinal tract. RESULTS: There was some evidence that long-term colonization led to genome degradation, but more evidence that Campylobacter adapted through the accumulation of non-synonymous single nucleotide polymorphisms (SNPs) and frameshifts in genes involved in cell motility, signal transduction and the major outer membrane protein (MOMP). The New Zealand patient also displayed considerable variation in their microbiome, inflammation and immunosuppression over five months, and the Campylobacter collected from this patient could be divided into two subpopulations, the proportion of which correlated with the amount of gastrointestinal inflammation. CONCLUSIONS: This study demonstrates how genomics, phylogenetics, 16S rRNA metabarcoding and biochemical markers can provide insight into how Campylobacter adapts to changing environments within human hosts. This study also demonstrates that long-term human colonization selects for changes in Campylobacter genes involved in cell motility, signal transduction and the MOMP; and that genetically distinct subpopulations of Campylobacter evolve to adapt to the changing gastrointestinal environment.
dc.description.confidentialfalse
dc.edition.editionDecember 2021
dc.format.pagination72-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/34893079
dc.identifier.citationBloomfield SJ, Midwinter AC, Biggs PJ, French NP, Marshall JC, Hayman DTS, Carter PE, Mather AE, Fayaz A, Thornley C, Kelly DJ, Benschop J. (2021). Genomic adaptations of Campylobacter jejuni to long-term human colonization.. Gut Pathog. 13. 1. (pp. 72-).
dc.identifier.doi10.1186/s13099-021-00469-7
dc.identifier.eissn1757-4749
dc.identifier.elements-typejournal-article
dc.identifier.issn1757-4749
dc.identifier.numberARTN 72
dc.identifier.pii10.1186/s13099-021-00469-7
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70627
dc.languageeng
dc.publisherBioMed Central Ltd
dc.publisher.urihttps://gutpathogens.biomedcentral.com/articles/10.1186/s13099-021-00469-7
dc.relation.isPartOfGut Pathog
dc.rights(c) The author/sen
dc.rights.licenseCC BY 4.0en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectCampylobacter
dc.subjectGenomics
dc.subjectHost adaptation
dc.subjectPhylogenetics
dc.titleGenomic adaptations of Campylobacter jejuni to long-term human colonization
dc.typeJournal article
pubs.elements-id450057
pubs.organisational-groupOther
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