Mapping immunogenic epitopes of an adhesin-like protein from Methanobrevibacter ruminantium M1 and comparison of empirical data with in silico prediction methods.

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dc.citation.issue1
dc.citation.volume12
dc.contributor.authorKhanum S
dc.contributor.authorCarbone V
dc.contributor.authorGupta SK
dc.contributor.authorYeung J
dc.contributor.authorShu D
dc.contributor.authorWilson T
dc.contributor.authorParlane NA
dc.contributor.authorAltermann E
dc.contributor.authorEstein SM
dc.contributor.authorJanssen PH
dc.contributor.authorWedlock DN
dc.contributor.authorHeiser A
dc.coverage.spatialEngland
dc.date.accessioned2024-08-15T02:33:39Z
dc.date.available2024-08-15T02:33:39Z
dc.date.issued2022-06-21
dc.description.abstractIn silico prediction of epitopes is a potentially time-saving alternative to experimental epitope identification but is often subject to misidentification of epitopes and may not be useful for proteins from archaeal microorganisms. In this study, we mapped B- and T-cell epitopes of a model antigen from the methanogen Methanobrevibacter ruminantium M1, the Big_1 domain (AdLP-D1, amino acids 19-198) of an adhesin-like protein. A series of 17 overlapping 20-mer peptides was selected to cover the Big_1 domain. Peptide-specific antibodies were produced in mice and measured by ELISA, while an in vitro splenocyte re-stimulation assay determined specific T-cell responses. Overall, five peptides of the 17 peptides were shown to be major immunogenic epitopes of AdLP-D1. These immunogenic regions were examined for their localization in a homology-based model of AdLP-D1. Validated epitopes were found in the outside region of the protein, with loop like secondary structures reflecting their flexibility. The empirical data were compared with epitope predictions made by programmes based on a range of algorithms. In general, the epitopes identified by in silico predictions were not comparable to those determined empirically.
dc.description.confidentialfalse
dc.format.pagination10394-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35729277
dc.identifier.citationKhanum S, Carbone V, Gupta SK, Yeung J, Shu D, Wilson T, Parlane NA, Altermann E, Estein SM, Janssen PH, Wedlock DN, Heiser A. (2022). Mapping immunogenic epitopes of an adhesin-like protein from Methanobrevibacter ruminantium M1 and comparison of empirical data with in silico prediction methods.. Sci Rep. 12. 1. (pp. 10394-).
dc.identifier.doi10.1038/s41598-022-14545-8
dc.identifier.eissn2045-2322
dc.identifier.elements-typejournal-article
dc.identifier.issn2045-2322
dc.identifier.pii10.1038/s41598-022-14545-8
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71313
dc.languageeng
dc.publisherSpringer Nature Limited
dc.publisher.urihttps://www.nature.com/articles/s41598-022-14545-8
dc.relation.isPartOfSci Rep
dc.rights(c) 2022 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAdhesins, Bacterial
dc.subjectAlgorithms
dc.subjectAnimals
dc.subjectEpitope Mapping
dc.subjectEpitopes, T-Lymphocyte
dc.subjectMethanobrevibacter
dc.subjectMice
dc.subjectPeptides
dc.titleMapping immunogenic epitopes of an adhesin-like protein from Methanobrevibacter ruminantium M1 and comparison of empirical data with in silico prediction methods.
dc.typeJournal article
pubs.elements-id486126
pubs.organisational-groupOther
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