Impact of Gut Recolonization on Liver Regeneration: Hepatic Matrisome Gene Expression after Partial Hepatectomy in Mice.

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dc.citation.issue13
dc.citation.volume24
dc.contributor.authorAmin AR
dc.contributor.authorHairulhisyam NM
dc.contributor.authorAqilah RNF
dc.contributor.authorNur Fariha MM
dc.contributor.authorMallard BL
dc.contributor.authorShanahan F
dc.contributor.authorWheatley AM
dc.contributor.authorMarlini M
dc.contributor.editorNeuman M
dc.contributor.editorMalnick S
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-06-16T23:46:11Z
dc.date.available2024-06-16T23:46:11Z
dc.date.issued2023-06-28
dc.description.abstractThe hepatic matrisome is involved in the remodeling phase of liver regeneration. As the gut microbiota has been implicated in liver regeneration, we investigated its role in liver regeneration focusing on gene expression of the hepatic matrisome after partial hepatectomy (PHx) in germ-free (GF) mice, and in GF mice reconstituted with normal gut microbiota (XGF). Liver mass restoration, hepatocyte proliferation, and immune response were assessed following 70% PHx. Hepatic matrisome and collagen gene expression were also analyzed. Reduced liver weight/body weight ratio, mitotic count, and hepatocyte proliferative index at 72 h post PHx in GF mice were preceded by reduced expression of cytokine receptor genes Tnfrsf1a and Il6ra, and Hgf gene at 3 h post PHx. In XGF mice, these indices were significantly higher than in GF mice, and similar to that of control mice, indicating normal liver regeneration. Differentially expressed genes (DEGs) of the matrisome were lower in GF compared to XGF mice at both 3 h and 72 h post PHx. GF mice also demonstrated lower collagen expression, with significantly lower expression of Col1a1, Col1a2, Col5a1, and Col6a2 compared to WT mice at 72 h post PHx. In conclusion, enhanced liver regeneration and matrisome expression in XGF mice suggests that interaction of the gut microbiota and matrisome may play a significant role in the regulation of hepatic remodeling during the regenerative process.
dc.description.confidentialfalse
dc.edition.editionJuly-1 2023
dc.format.pagination10774-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37445951
dc.identifier.citationAmin AR, Hairulhisyam NM, Aqilah RNF, Nur Fariha MM, Mallard BL, Shanahan F, Wheatley AM, Marlini M. (2023). Impact of Gut Recolonization on Liver Regeneration: Hepatic Matrisome Gene Expression after Partial Hepatectomy in Mice.. Int J Mol Sci. 24. 13. (pp. 10774-).
dc.identifier.doi10.3390/ijms241310774
dc.identifier.eissn1422-0067
dc.identifier.elements-typejournal-article
dc.identifier.issn1661-6596
dc.identifier.number10774
dc.identifier.piiijms241310774
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/69853
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/1422-0067/24/13/10774
dc.relation.isPartOfInt J Mol Sci
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectcollagen
dc.subjectgerm-free mice
dc.subjectgut microbiota
dc.subjectliver regeneration
dc.subjectmatrisome
dc.subjectAnimals
dc.subjectMice
dc.subjectHepatectomy
dc.subjectLiver Regeneration
dc.subjectLiver
dc.subjectGene Expression
dc.titleImpact of Gut Recolonization on Liver Regeneration: Hepatic Matrisome Gene Expression after Partial Hepatectomy in Mice.
dc.typeJournal article
pubs.elements-id479136
pubs.organisational-groupCollege of Health
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