Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis

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dc.citation.issue4
dc.citation.volume4
dc.contributor.authorPanizzi L
dc.contributor.authorVignes M
dc.contributor.authorDittmer KE
dc.contributor.authorWaterland MR
dc.contributor.authorRogers CW
dc.contributor.authorSano H
dc.contributor.authorMcIlwraith CW
dc.contributor.authorPemberton S
dc.contributor.authorOwen M
dc.contributor.authorRiley CB
dc.coverage.spatialEngland
dc.date.accessioned2023-12-15T01:22:35Z
dc.date.accessioned2024-07-25T06:37:14Z
dc.date.available2022-08-08
dc.date.available2023-12-15T01:22:35Z
dc.date.available2024-07-25T06:37:14Z
dc.date.issued2022-12
dc.description.abstractOBJECTIVE: to determine the accuracy of infrared (IR)-based serum biomarker profiling to differentiate horses with early inflammatory changes associated with a traumatically induced model of equine carpal osteoarthritis (OA) from controls. METHOD: unilateral carpal OA was induced in 9 of 17 healthy Thoroughbred fillies, while the remainder served as sham operated controls. Serum samples were obtained before induction of OA (Day 0) and weekly thereafter until Day 63 from both groups. Films of dried serum were created, and IR absorbance spectra acquired. Following pre-processing, partial least squares discriminant analysis (PLSDA) and principal component analysis (PCA) were used to assess group and time differences and generate predictive models for wavenumber ranges 1300-1800 ​cm-1 and 2600-3700  ​cm-1. RESULTS: the overall correct classification rate when classifying samples by group (OA or Sham) was 52.7% (s.d. ​= ​12.8%), while it was 94.0% (s.d. ​= ​1.4%) by sampling Day. The correct classification results by group-sampling Day combinations with pre-intervention serum (Day 0) was 50.5% (s.d. ​= ​21.7%). CONCLUSION: with the current approach IR spectroscopic analysis could not differentiate serum of horses with induced carpal OA from that of controls. The high classification rate obtained by Day of sampling may reflect the effect of exercise on the biomarker profile. A longer study period (advanced disease) or naturally occurring disease may provide further information on the suitability of this technique in horses.
dc.description.confidentialfalse
dc.edition.editionDecember 2022
dc.format.pagination100297-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36474792
dc.identifier.citationPanizzi L, Vignes M, Dittmer KE, Waterland MR, Rogers CW, Sano H, McIlwraith CW, Pemberton S, Owen M, Riley CB. (2022). Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis.. Osteoarthr Cartil Open. 4. 4. (pp. 100297-).
dc.identifier.doi10.1016/j.ocarto.2022.100297
dc.identifier.eissn2665-9131
dc.identifier.elements-typejournal-article
dc.identifier.issn2665-9131
dc.identifier.number100297
dc.identifier.piiS2665-9131(22)00065-6
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70552
dc.languageeng
dc.publisherElsevier Ltd on behalf of Osteoarthritis Research Society International (OARSI)
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S2665913122000656
dc.relation.isPartOfOsteoarthr Cartil Open
dc.rights(c) The author/sen
dc.rights.licenseCC BY-NC-Nen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBiomarker
dc.subjectEquine
dc.subjectOsteoarthritis
dc.subjectSerum
dc.subjectSpectroscopy
dc.titleInfrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis
dc.typeJournal article
pubs.elements-id458432
pubs.organisational-groupOther
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