Antibiotic and Heavy Metal Resistance in Bacteria from Contaminated Agricultural Soil: Insights from a New Zealand Airstrip

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dc.citation.issue2
dc.citation.volume14
dc.contributor.authorHeydari A
dc.contributor.authorKim ND
dc.contributor.authorBiggs PJ
dc.contributor.authorHorswell J
dc.contributor.authorGielen GJHP
dc.contributor.authorSiggins A
dc.contributor.authorBromhead C
dc.contributor.authorMeza-Alvarado JC
dc.contributor.authorPalmer BR
dc.contributor.editorAbia ALK
dc.coverage.spatialSwitzerland
dc.date.accessioned2025-03-10T01:22:25Z
dc.date.available2025-03-10T01:22:25Z
dc.date.issued2025-02
dc.description.abstractBACKGROUND/OBJECTIVES: Agricultural soils accumulate inorganic contaminants from the application of phosphate fertilisers. An airstrip located at Belmont Regional Park (BRP), near Wellington, New Zealand, has been found to have a gradient of cadmium contamination due to spillage of superphosphate fertiliser. METHODS: Soil samples from the BRP airstrip with a gradient of cadmium contamination, were used as a novel source to explore bacterial communities' resistance to heavy metals (HMs) and any co-selected antibiotic (Ab) resistance. RESULTS: Differences between BRP soil samples with higher levels of HMs compared to those with lower HM concentrations showed significantly more bacterial isolates resistant to both HMs (40.6% versus 63.1% resistant to 0.01 mM CdCl2, p < 0.05) and Abs (23.4% versus 37.8% resistant to 20 μg/mL tetracycline, p < 0.05) in soils with higher initial levels of HMs (1.14 versus 7.20 mg kg-1 Cd). Terminal restriction fragment length polymorphism (TRFLP) and 16S rDNA next-generation sequencing profiling investigated changes in HM-induced bacterial communities. Significant differences were observed among the bacterial community structures in the selected BRP soil samples. Conjugative transfer of cadmium resistance from 23-38% of cadmium-resistant isolates to a characterised recipient bacterial strain in vitro suggested many of these genes were carried by mobile genetic elements. Transconjugants were also resistant to zinc, mercury, and Abs. Higher levels of HMs in soil correlated with increased resistance to HMs, Abs, and elevated levels of HMs thus disturbed the bacterial community structure in BRP soil significantly. CONCLUSIONS: These findings suggest that HM contamination of agricultural soil can select for Ab resistance in soil bacteria with potential risks to human and animal health.
dc.description.confidentialfalse
dc.edition.editionFebruary 2025
dc.format.pagination192-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/40001435
dc.identifier.citationHeydari A, Kim ND, Biggs PJ, Horswell J, Gielen GJHP, Siggins A, Bromhead C, Meza-Alvarado JC, Palmer BR. (2025). Antibiotic and Heavy Metal Resistance in Bacteria from Contaminated Agricultural Soil: Insights from a New Zealand Airstrip.. Antibiotics (Basel). 14. 2. (pp. 192-).
dc.identifier.doi10.3390/antibiotics14020192
dc.identifier.eissn2079-6382
dc.identifier.elements-typejournal-article
dc.identifier.issn2079-6382
dc.identifier.number192
dc.identifier.piiantibiotics14020192
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/72606
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2079-6382/14/2/192
dc.relation.isPartOfAntibiotics (Basel)
dc.subjectPICT
dc.subjectantimicrobial resistance
dc.subjectbacteria
dc.subjectheavy metal resistance
dc.subjectsoil
dc.titleAntibiotic and Heavy Metal Resistance in Bacteria from Contaminated Agricultural Soil: Insights from a New Zealand Airstrip
dc.typeJournal article
pubs.elements-id499941
pubs.organisational-groupOther
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