Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium.

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dc.citation.issue1
dc.citation.volume13
dc.contributor.authorKumar S
dc.contributor.authorAltermann E
dc.contributor.authorLeahy SC
dc.contributor.authorJauregui R
dc.contributor.authorJonker A
dc.contributor.authorHenderson G
dc.contributor.authorKittelmann S
dc.contributor.authorAttwood GT
dc.contributor.authorKamke J
dc.contributor.authorWaters SM
dc.contributor.authorPatchett ML
dc.contributor.authorJanssen PH
dc.coverage.spatialEngland
dc.date.accessioned2024-08-27T21:17:40Z
dc.date.available2024-08-27T21:17:40Z
dc.date.issued2022-10-20
dc.description.abstractQuinella is a genus of iconic rumen bacteria first reported in 1913. There are no cultures of these bacteria, and information on their physiology is scarce and contradictory. Increased abundance of Quinella was previously found in the rumens of some sheep that emit low amounts of methane (CH4) relative to their feed intake, but whether Quinella contributes to low CH4 emissions is not known. Here, we concentrate Quinella cells from sheep rumen contents, extract and sequence DNA, and reconstruct Quinella genomes that are >90% complete with as little as 0.20% contamination. Bioinformatic analyses of the encoded proteins indicate that lactate and propionate formation are major fermentation pathways. The presence of a gene encoding a potential uptake hydrogenase suggests that Quinella might be able to use free hydrogen (H2). None of the inferred metabolic pathways is predicted to produce H2, a major precursor of CH4, which is consistent with the lower CH4 emissions from those sheep with high abundances of this bacterium.
dc.description.confidentialfalse
dc.edition.edition2022
dc.format.pagination6240-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36266280
dc.identifier.citationKumar S, Altermann E, Leahy SC, Jauregui R, Jonker A, Henderson G, Kittelmann S, Attwood GT, Kamke J, Waters SM, Patchett ML, Janssen PH. (2022). Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium.. Nat Commun. 13. 1. (pp. 6240-).
dc.identifier.doi10.1038/s41467-022-34013-1
dc.identifier.eissn2041-1723
dc.identifier.elements-typejournal-article
dc.identifier.issn2041-1723
dc.identifier.number6240
dc.identifier.pii10.1038/s41467-022-34013-1
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71388
dc.languageeng
dc.publisherNature Portfolio
dc.publisher.urihttps://www.nature.com/articles/s41467-022-34013-1
dc.relation.isPartOfNat Commun
dc.rights(c) 2022 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSheep
dc.subjectAnimals
dc.subjectRumen
dc.subjectPropionates
dc.subjectBacteria
dc.subjectMethane
dc.subjectFermentation
dc.subjectHydrogen
dc.subjectVeillonellaceae
dc.subjectGenomics
dc.subjectLactates
dc.subjectDiet
dc.titleGenomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium.
dc.typeJournal article
pubs.elements-id486125
pubs.organisational-groupOther
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