Antibacterial efficacy and possible mechanism of action of 2-hydroxyisocaproic acid (HICA)

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dc.citation.issue4
dc.citation.volume17
dc.contributor.authorPahalagedara ASNW
dc.contributor.authorFlint S
dc.contributor.authorPalmer J
dc.contributor.authorBrightwell G
dc.contributor.authorGupta TB
dc.contributor.editorNevárez-Moorillón GV
dc.coverage.spatialUnited States
dc.date.accessioned2023-10-24T22:32:13Z
dc.date.accessioned2023-10-25T20:49:31Z
dc.date.available2022-04
dc.date.available2023-10-24T22:32:13Z
dc.date.available2023-10-25T20:49:31Z
dc.date.issued2022-04-01
dc.date.updated2023-10-16T22:08:59Z
dc.description.abstractThe exploitation of natural antimicrobial compounds that can be used in food preservation has been fast tracked by the development of antimicrobial resistance to existing antimicrobials and the increasing consumer demand for natural food preservatives. 2-hydroxyisocaproic acid (HICA) is a natural compound produced through the leucine degradation pathway and is produced in humans and by certain microorganisms such as lactic acid bacteria and Clostridium species. The present study investigated the antibacterial efficacy of HICA against some important bacteria associated with food quality and safety and provided some insights into its possible antimicrobial mechanisms against bacteria. The results revealed that HICA was effective in inhibiting the growth of tested Gram-positive and Gram-negative bacteria including a multi-drug resistant P. aeruginosa strain in this study. The underlying mechanism was investigated by measuring the cell membrane integrity, membrane permeability, membrane depolarisation, and morphological and ultrastructural changes after HICA treatment in bacterial cells. The evidence supports that HICA exerts its activity via penetration of the bacterial cell membranes, thereby causing depolarisation, rupture of membranes, subsequent leakage of cellular contents and cell death. The current study suggests that HICA has potential to be used as an antibacterial agent against food spoilage and food-borne pathogenic bacteria, targeting the bacterial cell envelope.
dc.description.confidentialfalse
dc.format.extente0266406-
dc.identifiere0266406
dc.identifierPONE-D-21-34303
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/35363830
dc.identifier.citationPahalagedara ASNW, Flint S, Palmer J, Brightwell G, Gupta TB. (2022). Antibacterial efficacy and possible mechanism of action of 2-hydroxyisocaproic acid (HICA).. PLoS One. 17. 4. (pp. e0266406-).
dc.identifier.doi10.1371/journal.pone.0266406
dc.identifier.eissn1932-6203
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10179/20387
dc.languageeng
dc.publisherPLOS
dc.publisher.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0266406
dc.relation.isPartOfPLoS One
dc.rights(c) 2022 The Author/sen_US
dc.rightsCC BY 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectAnti-Bacterial Agents
dc.subjectAnti-Infective Agents
dc.subjectCaproates
dc.subjectGram-Negative Bacteria
dc.subjectGram-Positive Bacteria
dc.subjectHumans
dc.subjectMicrobial Sensitivity Tests
dc.titleAntibacterial efficacy and possible mechanism of action of 2-hydroxyisocaproic acid (HICA)
dc.typeJournal article
pubs.elements-id452640
pubs.organisational-groupOther
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