Bacterial lipopolysaccharide modulates immune response in the colorectal tumor microenvironment.

dc.citation.issue1
dc.citation.volume9
dc.contributor.authorSulit AK
dc.contributor.authorDaigneault M
dc.contributor.authorAllen-Vercoe E
dc.contributor.authorSilander OK
dc.contributor.authorHock B
dc.contributor.authorMcKenzie J
dc.contributor.authorPearson J
dc.contributor.authorFrizelle FA
dc.contributor.authorSchmeier S
dc.contributor.authorPurcell R
dc.coverage.spatialUnited States
dc.date.accessioned2024-08-22T21:15:34Z
dc.date.available2024-08-22T21:15:34Z
dc.date.issued2023-08-23
dc.description.abstractImmune responses can have opposing effects in colorectal cancer (CRC), the balance of which may determine whether a cancer regresses, progresses, or potentially metastasizes. These effects are evident in CRC consensus molecular subtypes (CMS) where both CMS1 and CMS4 contain immune infiltrates yet have opposing prognoses. The microbiome has previously been associated with CRC and immune response in CRC but has largely been ignored in the CRC subtype discussion. We used CMS subtyping on surgical resections from patients and aimed to determine the contributions of the microbiome to the pleiotropic effects evident in immune-infiltrated subtypes. We integrated host gene-expression and meta-transcriptomic data to determine the link between immune characteristics and microbiome contributions in these subtypes and identified lipopolysaccharide (LPS) binding as a potential functional mechanism. We identified candidate bacteria with LPS properties that could affect immune response, and tested the effects of their LPS on cytokine production of peripheral blood mononuclear cells (PBMCs). We focused on Fusobacterium periodonticum and Bacteroides fragilis in CMS1, and Porphyromonas asaccharolytica in CMS4. Treatment of PBMCs with LPS isolated from these bacteria showed that F. periodonticum stimulates cytokine production in PBMCs while both B. fragilis and P. asaccharolytica had an inhibitory effect. Furthermore, LPS from the latter two species can inhibit the immunogenic properties of F. periodonticum LPS when co-incubated with PBMCs. We propose that different microbes in the CRC tumor microenvironment can alter the local immune activity, with important implications for prognosis and treatment response.
dc.description.confidentialfalse
dc.edition.editionAugust 2023
dc.format.pagination59-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37612266
dc.identifier.citationSulit AK, Daigneault M, Allen-Vercoe E, Silander OK, Hock B, McKenzie J, Pearson J, Frizelle FA, Schmeier S, Purcell R. (2023). Bacterial lipopolysaccharide modulates immune response in the colorectal tumor microenvironment.. NPJ Biofilms Microbiomes. 9. 1. (pp. 59-).
dc.identifier.doi10.1038/s41522-023-00429-w
dc.identifier.eissn2055-5008
dc.identifier.elements-typejournal-article
dc.identifier.issn2055-5008
dc.identifier.number59
dc.identifier.pii10.1038/s41522-023-00429-w
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71367
dc.languageeng
dc.publisherNature Portfolio
dc.publisher.urihttps://www.nature.com/articles/s41522-023-00429-w
dc.relation.isPartOfNPJ Biofilms Microbiomes
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectHumans
dc.subjectLipopolysaccharides
dc.subjectLeukocytes, Mononuclear
dc.subjectTumor Microenvironment
dc.subjectBacteria
dc.subjectColorectal Neoplasms
dc.subjectCytokines
dc.subjectImmunity
dc.titleBacterial lipopolysaccharide modulates immune response in the colorectal tumor microenvironment.
dc.typeJournal article
pubs.elements-id479911
pubs.organisational-groupOther
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