Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and GreenshellTM Mussel Feeding on Plasma of Ovariectomized Rats

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dc.citation.issue11
dc.citation.volume11
dc.contributor.authorAbshirini M
dc.contributor.authorCabrera D
dc.contributor.authorFraser K
dc.contributor.authorSiriarchavatana P
dc.contributor.authorWolber FM
dc.contributor.authorMiller MR
dc.contributor.authorTian HS
dc.contributor.authorKruger MC
dc.contributor.editorWhitfield P
dc.contributor.editorRizzo M
dc.coverage.spatialSwitzerland
dc.date.accessioned2023-07-18T02:31:47Z
dc.date.accessioned2023-09-04T01:40:47Z
dc.date.available2021-10-31
dc.date.available2023-07-18T02:31:47Z
dc.date.available2023-09-04T01:40:47Z
dc.date.issued2021-10-31
dc.date.updated2023-07-16T23:50:33Z
dc.description(c) The Author/sen_US
dc.description.abstractThis study aimed to examine the changes in lipid and metabolite profiles of ovariectomized (OVX) rats with diet-induced metabolic syndrome-associated osteoarthritis (MetOA) after supplementation with greenshell mussel (GSM) using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach. Ninety-six rats were fed with one of four diets: control, control supplemented with GSM + GSM, high fat/high sugar (HFHS), or high fat/high sugar enriched with GSM (HFHS + GSM). After 8 weeks on experimental diets, half of the rats in each group underwent OVX and the other half were sham operated. After being fed for an additional 28 weeks, blood samples were collected for the metabolomics analysis. Lipid and polar metabolites were extracted from plasma and analysed by LC-MS. We identified 29 lipid species from four lipid subclasses (phosphatidylcholine, lysophosphatidylcholine, diacylglycerol, and triacylglycerol) and a set of eight metabolites involved in amino acid metabolism (serine, threonine, lysine, valine, histidine, pipecolic acid, 3-methylcytidine, and cholic acid) as potential biomarkers for the effect of HFHS diet and GSM supplementation. GSM incorporation more specifically in the control diet generated significant alterations in the levels of several lipids and metabolites. Further studies are required to validate these findings that identify potential biomarkers to follow OA progression and to monitor the impact of GSM supplementation.
dc.description.confidentialfalse
dc.edition.editionNovember 2021
dc.format.extent754-
dc.identifierARTN 754
dc.identifiermetabo11110754
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/34822412
dc.identifier.citationAbshirini M, Cabrera D, Fraser K, Siriarchavatana P, Wolber FM, Miller MR, Tian HS, Kruger MC. (2021). Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and GreenshellTM Mussel Feeding on Plasma of Ovariectomized Rats.. Metabolites. 11. 11. (pp. 754-).
dc.identifier.doi10.3390/metabo11110754
dc.identifier.eissn2218-1989
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2218-1989
dc.identifier.urihttp://hdl.handle.net/10179/19878
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2218-1989/11/11/754
dc.relation.isPartOfMetabolites
dc.rights.uriCC BY 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectdiet-induced obese rats
dc.subjectlipidomic
dc.subjectmetabolic dysregulation
dc.subjectmetabolomics
dc.subjectosteoarthritis
dc.titleMass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and GreenshellTM Mussel Feeding on Plasma of Ovariectomized Rats
dc.typeJournal article
pubs.elements-id449714
pubs.organisational-groupOther
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