An accumulated mutation gained in mosquito cells enhances Zika virus virulence and fitness in mice.

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dc.citation.volumeOnline First
dc.contributor.authorFan X-X
dc.contributor.authorLi R-T
dc.contributor.authorZhu Y-B
dc.contributor.authorChen Q
dc.contributor.authorLi X-F
dc.contributor.authorCao T-S
dc.contributor.authorZhao H
dc.contributor.authorCheng G
dc.contributor.authorQin C-F
dc.contributor.editorHeise MT
dc.coverage.spatialUnited States
dc.date.accessioned2024-11-12T00:15:21Z
dc.date.available2024-11-12T00:15:21Z
dc.date.issued2024-10-16
dc.description.abstractZika virus (ZIKV) remains a significant public health threat worldwide. A number of adaptive mutations have accumulated within the genome of ZIKV during global transmission, some of which have been linked to specific phenotypes. ZIKV maintains an alternating cycle of replication between mosquitoes and vertebrate hosts, but the role of mosquito-specific adaptive mutations in ZIKV has not been well investigated. In this study, we demonstrated that serial passaging of ZIKV in mosquito Aag2 cells led to the emergence of critical amino acid substitutions, including A94V in the prM protein and V153D and H401Y in the E protein. Further characterization via reverse genetics revealed that the H401Y substitution in the E protein did not augment viral replication in mosquitoes but significantly enhanced neurovirulence and lethality compared with those of the wild-type (WT) virus in mice. More importantly, the H401Y mutant maintained its virulence phenotype in mice after propagation in mosquitoes in mosquito-mouse cycle model. In particular, recombinant ZIKV harboring the H401Y substitution showed enhanced competitive fitness over WT ZIKV in various mammalian cells and mouse brains, but not in mosquito cells. Notably, the H401Y substitution in the ZIKV E protein has been detected in recent isolates derived from both mosquitoes and humans in Asia and the Americas. In summary, our findings not only identify a novel virulence determinant of ZIKV but also highlight the complexity of the relationship between the evolution of vector-borne viruses and their clinical outcome in nature.
dc.description.confidentialfalse
dc.edition.editionOct 2024
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/39412258
dc.identifier.citationFan X-X, Li R-T, Zhu Y-B, Chen Q, Li X-F, Cao T-S, Zhao H, Cheng G, Qin C-F. (2024). An accumulated mutation gained in mosquito cells enhances Zika virus virulence and fitness in mice.. Journal of Virology. Online First.
dc.identifier.doi10.1128/jvi.01251-24
dc.identifier.eissn1098-5514
dc.identifier.elements-typejournal-article
dc.identifier.issn0022-538X
dc.identifier.numbere0125124
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71975
dc.languageEnglish
dc.publisherAmerican Society for Microbiology
dc.publisher.urihttps://journals.asm.org/doi/10.1128/jvi.01251-24
dc.relation.isPartOfJournal of Virology
dc.rights(c) 2024 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectaccumulated mutation
dc.subjectfitness
dc.subjectmammal
dc.subjectmosquito
dc.subjectvirulence
dc.subjectZika virus
dc.titleAn accumulated mutation gained in mosquito cells enhances Zika virus virulence and fitness in mice.
dc.typeJournal article
pubs.elements-id492012
pubs.organisational-groupCollege of Health
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