The characterisation of antimicrobial resistant Escherichia coli from dairy calves.

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dc.citation.issue8
dc.citation.volume72
dc.contributor.authorMwenifumbo M
dc.contributor.authorCookson AL
dc.contributor.authorZhao S
dc.contributor.authorFayaz A
dc.contributor.authorBrowne AS
dc.contributor.authorBenschop J
dc.contributor.authorBurgess SA
dc.coverage.spatialEngland
dc.date.accessioned2024-07-30T01:34:18Z
dc.date.available2024-07-30T01:34:18Z
dc.date.issued2023-08
dc.description.abstractIntroduction. Dairy calves, particularly pre-weaned calves have been identified as a common source of multidrug resistant (MDR) Escherichia coli. Gap statement. E. coli strains isolated from dairy calves and the location of their resistance genes (plasmid or chromosomal) have not been well characterised. Aim. To characterise the phenotypic and genotypic features as well as the population structure of antimicrobial-resistant E. coli isolated from calves located on dairy farms that feed waste-milk to their replacement calves. Methodology. Recto-anal swab enrichments from 40 dairy calves (≤ 14 days old) located on four dairy farms were examined for tetracycline, streptomycin, ciprofloxacin, and third-generation cephalosporin resistant E. coli. Whole genome sequencing was performed using both short- and long-read technologies on selected antimicrobial resistant E. coli. Results. Fifty-eight percent (23/40) of calves harboured antimicrobial resistant E. coli: 43 % (17/40) harboured tetracycline resistant, and 23 % (9/40) harboured chromosomal mediated AmpC producing E. coli. Whole genome sequencing of 27 isolates revealed five sequence types, with ST88 being the dominant ST (17/27, 63 % of the sequenced isolates) followed by ST1308 (3/27, 11 %), along with the extraintestinal pathogenic E. coli lineages ST69 (3/27, 11 %), ST10 (2/27, 7 %), and ST58 (2/27, 7 %). Additionally, 16 isolates were MDR, harbouring additional resistance genes that were not tested phenotypically. Oxford Nanopore long-read sequencing technologies enabled the location of multiple resistant gene cassettes in IncF plasmids to be determined. Conclusion. Our study identified a high incidence of tetracycline and streptomycin-resistant E. coli in dairy calves, and highlighted the presence of multidrug-resistant strains, emphasising the need for further investigation into potential associations with farm management practices.
dc.description.confidentialfalse
dc.edition.edition2023
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37578342
dc.identifier.citationMwenifumbo M, Cookson AL, Zhao S, Fayaz A, Browne AS, Benschop J, Burgess SA. (2023). The characterisation of antimicrobial resistant Escherichia coli from dairy calves.. J Med Microbiol. 72. 8.
dc.identifier.doi10.1099/jmm.0.001742
dc.identifier.eissn1473-5644
dc.identifier.elements-typejournal-article
dc.identifier.issn0022-2615
dc.identifier.number001742
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71145
dc.languageeng
dc.publisherMicrobiology Society
dc.publisher.urihttps://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001742
dc.relation.isPartOfJ Med Microbiol
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectEscherichia coli
dc.subjectantimicrobial resistance
dc.subjectcalves
dc.subjectdairy
dc.subjectAnimals
dc.subjectCattle
dc.subjectEscherichia coli
dc.subjectAnti-Infective Agents
dc.subjectAnti-Bacterial Agents
dc.subjectEscherichia coli Infections
dc.subjectTetracycline
dc.subjectStreptomycin
dc.titleThe characterisation of antimicrobial resistant Escherichia coli from dairy calves.
dc.typeJournal article
pubs.elements-id479833
pubs.organisational-groupOther
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