Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden

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dc.citation.volume12
dc.contributor.authorBai X
dc.contributor.authorZhang J
dc.contributor.authorHua Y
dc.contributor.authorJernberg C
dc.contributor.authorXiong Y
dc.contributor.authorFrench N
dc.contributor.authorLöfgren S
dc.contributor.authorHedenström I
dc.contributor.authorAmbikan A
dc.contributor.authorMernelius S
dc.contributor.authorMatussek A
dc.contributor.editorGonzález-Escalona N
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-01-25T00:54:57Z
dc.date.accessioned2024-07-25T06:39:02Z
dc.date.available2021-02-05
dc.date.available2024-01-25T00:54:57Z
dc.date.available2024-07-25T06:39:02Z
dc.date.issued2021-02-05
dc.description.abstractShiga toxin-producing Escherichia coli (STEC) are important foodborne pathogens that can cause human infections ranging from asymptomatic carriage to bloody diarrhea (BD) and fatal hemolytic uremic syndrome (HUS). However, the molecular mechanism of STEC pathogenesis is not entirely known. Here, we demonstrated a large scale of molecular epidemiology and in-depth genomic study of clinical STEC isolates utilizing clinical and epidemiological data collected in Region Jönköping County, Sweden, over a 15-year period. Out of 184 STEC isolates recovered from distinct patients, 55 were from patients with BD, and 129 were from individuals with non-bloody stools (NBS). Five individuals developed HUS. Adults were more associated with BD. Serotypes O157:H7, O26:H11, O103:H2, O121:H19, and O104:H4 were more often associated with BD. The presence of Shiga toxin-encoding gene subtypes stx 2a, stx 2a + stx 2c, and stx 1a + stx 2c was associated with BD, while stx 1 a was associated with milder disease. Multiplex virulence and accessory genes were correlated with BD; these genes encode toxins, adhesion, autotransporters, invasion, and secretion system. A number of antimicrobial resistance (AMR) genes, such as aminoglycoside, aminocoumarin, macrolide, and fluoroquinolone resistance genes, were prevalent among clinical STEC isolates. Whole-genome phylogeny revealed that O157 and non-O157 STEC isolates evolved from distinct lineages with a few exceptions. Isolates from BD showed more tendency to cluster closely. In conclusion, this study unravels molecular trait of clinical STEC strains and identifies genetic factors associated with severe clinical outcomes, which could contribute to management of STEC infections and disease progression if confirmed by further functional validation.
dc.description.confidentialfalse
dc.format.pagination627861-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33613494
dc.identifier.citationBai X, Zhang J, Hua Y, Jernberg C, Xiong Y, French N, Löfgren S, Hedenström I, Ambikan A, Mernelius S, Matussek A. (2021). Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden.. Front Microbiol. 12. (pp. 627861-).
dc.identifier.doi10.3389/fmicb.2021.627861
dc.identifier.eissn1664-302X
dc.identifier.elements-typejournal-article
dc.identifier.issn1664-302X
dc.identifier.numberARTN 627861
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70612
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2021.627861/full
dc.relation.isPartOfFront Microbiol
dc.subjectShiga toxin-producing Escherichia coli
dc.subjectbloody diarrhea
dc.subjectclinical outcomes
dc.subjectcomparative genomics
dc.subjectduration of bacterial shedding
dc.subjectwhole genome sequencing
dc.titleGenomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden
dc.typeJournal article
pubs.elements-id440925
pubs.organisational-groupOther
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