Phenotypic properties and genotyping analysis of Bacillus cereus group isolates from dairy and potato products

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dc.citation.volume140
dc.contributor.authorHuang Y
dc.contributor.authorFlint SH
dc.contributor.authorYu S
dc.contributor.authorDing Y
dc.contributor.authorPalmer JS
dc.date.accessioned2023-10-24T00:47:59Z
dc.date.accessioned2023-10-25T20:49:29Z
dc.date.available2021-01-05
dc.date.available2023-10-24T00:47:59Z
dc.date.available2023-10-25T20:49:29Z
dc.date.issued2021-04
dc.date.updated2023-10-16T20:11:28Z
dc.description.abstractBacillus cereus group (B. cereus sensu lato) are ubiquitously distributed in diverse environments. In this study, eight isolates including B. cereus, B. paranthracis and B. toyonensis species, from dairy and potato products, were assessed for biofilm formation, sporulation and genetic information including biofilm-related genes and toxin genes. The isolates varied in their ability to form biofilm (either at the stainless steel-liquid-air interface or floating pellicles). The amounts of biofilms of B. cereus s.l., were increased when incubated in agitation condition varied between isolates. Sporulation within the planktonic and biofilm modes of growth was compared, suggesting that biofilm is a favourable environment for B. cereus s.l. to form spores. Whole genome sequencing (WGS) was used to compare these B. cereus s.l. isolates. New sequence types (STs) of B. cereus were found in this study. Isolates that shared similar genomes had different biofilm-forming and sporulation abilities. Most of isolates tested, possessed biofilm-related genes. Different combinations of toxin-producing genes were identified in different isolates, with all isolates containing nhe while only some contained hbl and cytK. None of the food isolates contained the emetic ces gene. This study highlights the diversity of B. cereus s.l. in biofilm formation, sporulation and their genetic variables.
dc.description.confidentialfalse
dc.edition.editionApril 2021
dc.identifierARTN 110853
dc.identifierS0023643821000062
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000636112600096&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifier.citationHuang Y, Flint SH, Yu S, Ding Y, Palmer JS. (2021). Phenotypic properties and genotyping analysis of Bacillus cereus group isolates from dairy and potato products. LWT. 140.
dc.identifier.doi10.1016/j.lwt.2021.110853
dc.identifier.eissn1096-1127
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0023-6438
dc.identifier.urihttp://hdl.handle.net/10179/20383
dc.languageEnglish
dc.publisherElsevier Ltd
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0023643821000062
dc.relation.isPartOfLWT
dc.rightsCC BY 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectBacillus cereus
dc.subjectBiofilms
dc.subjectSporulation
dc.subjectBiofilm-related genes
dc.subjectToxin genes
dc.subjectWGS
dc.titlePhenotypic properties and genotyping analysis of Bacillus cereus group isolates from dairy and potato products
dc.typeJournal article
pubs.elements-id436747
pubs.organisational-groupOther
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