The deconstruction of pectic rhamnogalacturonan I unmasks the occurrence of a novel arabinogalactan oligosaccharide epitope

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dc.citation.issue11
dc.citation.volume56
dc.contributor.authorBuffetto F
dc.contributor.authorCornuault VRG
dc.contributor.authorRydahl MG
dc.contributor.authorRopartz D
dc.contributor.authorAlvarado C
dc.contributor.authorEchasserieau V
dc.contributor.authorLe Gall S
dc.contributor.authorBouchet B
dc.contributor.authorTranquet O
dc.contributor.authorVerhertbruggen Y
dc.contributor.authorWillats WGT
dc.contributor.authorKnox JP
dc.contributor.authorRalet MC
dc.contributor.authorGuillon F
dc.date.available1/11/2015
dc.date.issued1/11/2015
dc.description.abstractRhamnogalacturonan I (RGI) is a pectic polysaccharide composed of a backbone of alternating rhamnose and galacturonic acid residues with side chains containing galactose and/or arabinose residues. The structure of these side chains and the degree of substitution of rhamnose residues are extremely variable and depend on species, organs, cell types and developmental stages. Deciphering RGI function requires extending the current set of monoclonal antibodies (mAbs) directed to this polymer. Here, we describe the generation of a new mAb that recognizes a heterogeneous subdomain of RGI. The mAb, INRA-AGI-1, was produced by immunization of mice with RGI oligosaccharides isolated from potato tubers. These oligomers consisted of highly branched RGI backbones substituted with short side chains. INRA-AGI-1 bound specifically to RGI isolated from galactan-rich cell walls and displayed no binding to other pectic domains. In order to identify its RGI-related epitope, potato RGI oligosaccharides were fractionated by anion-exchange chromatography. Antibody recognition was assessed for each chromatographic fraction. INRA-AGI-1 recognizes a linear chain of (1→4)-linked galactose and (1→5)-linked arabinose residues. By combining the use of INRA-AGI-1 with LM5, LM6 and INRA-RU1 mAbs and enzymatic pre-treatments, evidence is presented of spatial differences in RGI motif distribution within individual cell walls of potato tubers and carrot roots. These observations raise questions about the biosynthesis and assembly of pectin structural domains and their integration and remodeling in cell walls.
dc.description.confidentialFALSE
dc.format.extent2181 - 2196
dc.identifier.citationPlant and Cell Physiology, 2015, 56 (11), pp. 2181 - 2196
dc.identifier.doi10.1093/pcp/pcv128
dc.identifier.elements-id403210
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0032-0781
dc.identifier.urihttps://hdl.handle.net/10179/13068
dc.languageenglish
dc.publisherOxford University Press (OUP)
dc.relation.isPartOfPlant and Cell Physiology
dc.relation.urihttps://academic.oup.com/pcp/article/56/11/2181/2755901
dc.subjectAG-I
dc.subjectINRA-AGI
dc.subjectplant cell wall
dc.subjectrhamnogalacturonan I
dc.subjectSolanum tuberosum
dc.subject.anzsrc0601 Biochemistry and Cell Biology
dc.subject.anzsrc0607 Plant Biology
dc.titleThe deconstruction of pectic rhamnogalacturonan I unmasks the occurrence of a novel arabinogalactan oligosaccharide epitope
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
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