Optical microlever assisted DNA stretching

Simple item page
dc.citation.issue16
dc.citation.volume29
dc.contributor.authorAndrew P-K
dc.contributor.authorRaudsepp A
dc.contributor.authorFan D
dc.contributor.authorStaufer U
dc.contributor.authorWilliams MAK
dc.contributor.authorAvci E
dc.coverage.spatialUnited States
dc.date.accessioned2024-01-28T20:29:50Z
dc.date.accessioned2024-07-25T06:49:11Z
dc.date.available2021-07-28
dc.date.available2024-01-28T20:29:50Z
dc.date.available2024-07-25T06:49:11Z
dc.date.issued2021-08-02
dc.description.abstractOptical microrobotics is an emerging field that has the potential to improve upon current optical tweezer studies through avenues such as limiting the exposure of biological molecules of interest to laser radiation and overcoming the current limitations of low forces and unwanted interactions between nearby optical traps. However, optical microrobotics has been historically limited to rigid, single-body end-effectors rather than even simple machines, limiting the tasks that can be performed. Additionally, while multi-body machines such as microlevers exist in the literature, they have not yet been successfully demonstrated as tools for biological studies, such as molecule stretching. In this work we have taken a step towards moving the field forward by developing two types of microlever, produced using two-photon absorption polymerisation, to perform the first lever-assisted stretches of double-stranded DNA. The aim of the work is to provide a proof of concept for using optical micromachines for single molecule studies. Both styles of microlevers were successfully used to stretch single duplexes of DNA, and the results were analysed with the worm-like chain model to show that they were in good agreement.
dc.description.confidentialfalse
dc.format.pagination25836-25847
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/34614903
dc.identifier.citationAndrew P-K, Raudsepp A, Fan D, Staufer U, Williams MAK, Avci E. (2021). Optical microlever assisted DNA stretching.. Opt Express. 29. 16. (pp. 25836-25847).
dc.identifier.doi10.1364/OE.430465
dc.identifier.eissn1094-4087
dc.identifier.elements-typejournal-article
dc.identifier.issn1094-4087
dc.identifier.pii453807
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70949
dc.languageeng
dc.publisherOptica Publishing Group
dc.publisher.urihttps://opg.optica.org/oe/fulltext.cfm?uri=oe-29-16-25836&id=453807
dc.relation.isPartOfOpt Express
dc.rights(c) The author/s (c) The Publishersen
dc.rights.licenseCC BY 4.0en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectDNA
dc.subjectNucleic Acid Conformation
dc.subjectOptical Tweezers
dc.subjectOptics and Photonics
dc.subjectProof of Concept Study
dc.subjectRobotics
dc.titleOptical microlever assisted DNA stretching
dc.typeJournal article
pubs.elements-id447967
pubs.organisational-groupOther
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