Bovine viral diarrhoea viruses from New Zealand belong predominantly to the BVDV-1a genotype.
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Date
2024-03-01
Open Access Location
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor and Francis Group
Rights
(c) 2023 The Author/s
CC BY 4.0
CC BY 4.0
Abstract
AIM: To determine which genotypes of bovine viral diarrhoea virus (BVDV) circulate among cattle in New Zealand.
METHODS: Samples comprised BVDV-1-positive sera sourced from submissions to veterinary diagnostic laboratories in 2019 (n = 25), 2020 (n = 59) and 2022 (n = 74) from both beef and dairy herds, as well as archival BVDV-1 isolates (n = 5). Fragments of the 5' untranslated region (5' UTR) and glycoprotein E2 coding sequence of the BVDV genome were amplified and sequenced. The sequences were aligned to each other and to international BVDV-1 sequences to determine their similarities and phylogenetic relationships. The 5' UTR sequences were also used to create genetic haplotype networks to determine if they were correlated with selected traits (location, type of farm, and year of collection).
RESULTS: The 5' UTR sequences from New Zealand BVDV were closely related to each other, with pairwise identities between 89% and 100%. All clustered together and were designated as BVDV-1a (n = 144) or BVDV-1c (n = 5). There was no evidence of a correlation between the 5' UTR sequence and the geographical origin within the country, year of collection or the type of farm. Partial E2 sequences from New Zealand BVDV (n = 76) showed 74-100% identity to each other and clustered in two main groups. The subtype assignment based on the E2 sequence was the same as based on the 5' UTR analysis. This is the first comprehensive analysis of genomic variability of contemporary New Zealand BVDV based on the analysis of the non-coding (5' UTR) and coding (E2) sequences.
CONCLUSIONS AND CLINICAL RELEVANCE: Knowledge of the diversity of the viruses circulating in the country is a prerequisite for the development of effective control strategies, including a selection of suitable vaccines. The data presented suggest that New Zealand BVDV are relatively homogeneous, which should facilitate eradication efforts including selection or development of the most suitable vaccines.
Description
Keywords
BVD virus, Bovine viral diarrhoea, New Zealand cattle, genetic diversity, phylogenetic analysis, Cattle, Animals, Diarrhea Viruses, Bovine Viral, Phylogeny, 5' Untranslated Regions, New Zealand, Bovine Virus Diarrhea-Mucosal Disease, Diarrhea Virus 1, Bovine Viral, Genotype, Vaccines, Cattle Diseases
Citation
Dunowska M, Lal R, Dissanayake SD, Bond SD, Burrows E, Moffat J, Howe L. (2024). Bovine viral diarrhoea viruses from New Zealand belong predominantly to the BVDV-1a genotype.. N Z Vet J. 72. 2. (pp. 66-78).