LM6-M: A high avidity rat monoclonal antibody to pectic α-1,5-L-arabinan

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dc.contributor.authorCornuault VRG
dc.contributor.authorBuffetto F
dc.contributor.authorMarcus SE
dc.contributor.authorCrepeau MJ
dc.contributor.authorGuillon F
dc.contributor.authorRalet MC
dc.contributor.authorKnox JP
dc.date.available10/07/2017
dc.date.issued10/07/2017
dc.description.abstract1,5-arabinan is an abundant structural feature of side chains of pectic rhamnogalacturonan-I which is a matrix constituent of plant cell walls. The study of arabinan in cells and tissues is driven by putative roles for this polysaccharide in the generation of cell wall and organ mechanical properties. The biological function(s) of arabinan is still uncertain and high quality molecular tools are required to detect its occurrence and monitor its dynamics. Here we report a new rat monoclonal antibody, LM6-M, similar in specificity to the published rat monoclonal antibody LM6 (Willats et al. (1998) Carbohydrate Research 308: 149-152). LM6-M is of the IgM immunoglobulin class and has a higher avidity for α-1-5-L-arabinan than LM6. LM6-M displays high sensitivity in its detection of arabinan in in-vitro assays such as ELISA and epitope detection chromatography and in in-situ analyses.
dc.identifier.citation2017
dc.identifier.doi10.1101/161604
dc.identifier.elements-id403286
dc.identifier.harvestedMassey_Dark
dc.identifier.urihttps://hdl.handle.net/10179/13071
dc.relation.urihttps://www.biorxiv.org/content/early/2017/07/10/161604
dc.subjectmonoclonal antibody
dc.subjectpectin
dc.subjectarabinan
dc.subjectrhamnogalacturonan
dc.subjectplant cell wall
dc.titleLM6-M: A high avidity rat monoclonal antibody to pectic α-1,5-L-arabinan
dc.typeinternet
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
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