Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis

Simple item page
dc.citation.issue1
dc.citation.volume12
dc.contributor.authorDittmer KE
dc.contributor.authorChernyavtseva A
dc.contributor.authorMarshall JC
dc.contributor.authorCabrera D
dc.contributor.authorWolber FM
dc.contributor.authorKruger M
dc.contributor.editorBienko M
dc.contributor.editorRadzki RP
dc.coverage.spatialSwitzerland
dc.date.accessioned2023-07-18T03:25:01Z
dc.date.accessioned2023-09-04T01:40:44Z
dc.date.available2021-12-29
dc.date.available2023-07-18T03:25:01Z
dc.date.available2023-09-04T01:40:44Z
dc.date.issued2022-01
dc.date.updated2023-07-13T00:16:30Z
dc.description(c) 2021 by the authors.en_US
dc.description.abstractOsteoporosis is a significant public health issue around the world, with post-menopausal osteoporosis due to estrogen deficiency resulting in approximately ¾ of cases. In this study, 18 aged Merino ewes were ovariectomized, and 10 were controls. Three of the ovariectomized ewes were treated weekly with 400 mg of methylprednisolone for 5 months and three were treated weekly for 2 months, followed by a 3-month recovery period. At 2 months, five control animals and six ovariectomized animals were euthanized. At 5 months, all the remaining ewes were euthanized. Kidney samples were collected postmortem for qPCR analysis of NPT1, PTH1R, NPT2a, NPT2c, Klotho, FGFR1IIIc, VDR, CYP24A1, CYP27B1, TRPV5, TRPV6, CalD9k, CalD28k, PMCA and NCX1. Ovariectomized sheep had significantly greater VDR expression compared with other groups. Ovariectomized sheep treated with glucocorticoids for 2 months followed by euthanasia at 5 months showed significant differences in TRPV5, CYP24A1 and klotho gene expression compared to other groups. Differences in klotho expression were most marked after adjustment for repeated measures (p = 0.1). Klotho is known as the "anti-aging" hormone and is involved in calcium and phosphorus metabolism. Klotho may be involved in the recovery of bone mineral density in ovariectomized sheep treated with glucocorticoids for 2 months followed by euthanasia at 5 months. Further research on the role of klotho is recommended.
dc.description.confidentialfalse
dc.edition.editionJanuary 2022
dc.format.extent67-
dc.identifierARTN 67
dc.identifierani12010067
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/35011173
dc.identifier.citationDittmer KE, Chernyavtseva A, Marshall JC, Cabrera D, Wolber FM, Kruger M. (2021). Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis.. Animals (Basel). 12. 1. (pp. 67-).
dc.identifier.doi10.3390/ani12010067
dc.identifier.eissn2076-2615
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2076-2615
dc.identifier.urihttp://hdl.handle.net/10179/19872
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2076-2615/12/1/67
dc.relation.isPartOfAnimals (Basel)
dc.rightsCC BYen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectcalcium metabolism
dc.subjectklotho
dc.subjectosteoporosis
dc.subjectphosphatonin
dc.subjectphosphorus metabolism
dc.subjectqPCR
dc.subjectsheep
dc.subjectvitamin D
dc.titleExpression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis
dc.typeJournal article
pubs.elements-id450265
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
450265 PDF.pdf
Size:
1.85 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles