Structural and Physicochemical Characteristics of Oil Bodies from Hemp Seeds (Cannabis sativa L.)

Simple item page
dc.citation.issue12
dc.citation.volume10
dc.contributor.authorGarcia FL
dc.contributor.authorMa S
dc.contributor.authorDave A
dc.contributor.authorAcevedo-Fani A
dc.contributor.editorFiorini D
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-05-20T03:17:52Z
dc.date.available2024-05-20T03:17:52Z
dc.date.issued2021-11-26
dc.description.abstractThe structural and physicochemical characteristics of oil bodies from hemp seeds were explored in this study. Oil bodies from several plant-based sources have been previously studied; however, this is the first time a characterisation of oil bodies from the seeds of industrial hemp is provided. The morphology of oil bodies in hemp seeds and after extraction was investigated using cryo-scanning electron microscopy (cryo-SEM), and the interfacial characteristics of isolated oil bodies were studied by confocal laser scanning microscopy (CLSM). Proteins associated with oil bodies were characterised using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The effect of pH and ionic strength on colloidal properties of the oil bodies was investigated. Oil bodies in hemp seeds appeared spherical and sporadically distributed in the cell, with diameters of 3 to 5 μm. CLSM images of isolated oil bodies revealed the uniform distribution of phospholipids and proteins at their interface. Polyunsaturated fatty acids were predominant in the lipid fraction and linoleic acid accounted for ≈61% of the total fatty acids. The SDS-PAGE analysis of washed and purified oil bodies revealed major bands at 15 kDa and 50-25 kDa, which could be linked to membrane-specific proteins of oil bodies or extraneous proteins. The colloidal stability of oil bodies in different pH environments indicated that the isoelectric point was between pH 4 and 4.5, where oil bodies experienced maximum aggregation. Changes in the ionic strength decreased the interfacial charge density of oil bodies (ζ-potential), but it did not affect their mean particle size. This suggested that the steric hindrance provided by membrane-specific proteins at the interface of the oil bodies could have prevented them from flocculation at low interfacial charge density. The results of this study provide new tertiary knowledge on the structure, composition, and colloidal properties of oil bodies extracted from hemp seeds, which could be used as natural emulsions or lipid-based delivery systems for food products.
dc.description.confidentialfalse
dc.edition.editionDecember 2021
dc.format.pagination2930-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/34945481
dc.identifier.citationGarcia FL, Ma S, Dave A, Acevedo-Fani A. (2021). Structural and Physicochemical Characteristics of Oil Bodies from Hemp Seeds (Cannabis sativa L.).. Foods. 10. 12. (pp. 2930-).
dc.identifier.doi10.3390/foods10122930
dc.identifier.eissn2304-8158
dc.identifier.elements-typejournal-article
dc.identifier.issn2304-8158
dc.identifier.numberARTN 2930
dc.identifier.piifoods10122930
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/69613
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2304-8158/10/12/2930
dc.relation.isPartOfFoods
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectdelivery systems
dc.subjecthempseeds
dc.subjectoil bodies
dc.subjectoleosomes
dc.subjectphysicochemical properties
dc.subjectstructure
dc.titleStructural and Physicochemical Characteristics of Oil Bodies from Hemp Seeds (Cannabis sativa L.)
dc.typeJournal article
pubs.elements-id450084
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Published version.pdf
Size:
4.97 MB
Format:
Adobe Portable Document Format
Description:
450084 PDF.pdf
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
9.22 KB
Format:
Plain Text
Description:
Download
Collections
Journal Articles