Theileria orientalis Ikeda infection detected in red deer but not dogs or horses in New Zealand.

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dc.contributor.authorLawrence KE
dc.contributor.authorGedye K
dc.contributor.authorCarvalho L
dc.contributor.authorWang B
dc.contributor.authorFermin LM
dc.contributor.authorPomroy WE
dc.coverage.spatialEngland
dc.date.accessioned2024-09-23T20:40:03Z
dc.date.available2024-09-23T20:40:03Z
dc.date.issued2024-09-02
dc.description.abstractAIMS: To determine whether evidence for infection with Theileria orientalis (Ikeda) could be identified in samples of commercial red deer (Cervus elaphus), horses, and working farm dogs in New Zealand. METHODS: Blood samples were collected during October and November 2019 from a convenience sample of red deer (n = 57) at slaughter. Equine blood samples (n = 50) were convenience-sampled from those submitted to a veterinary pathology laboratory for routine testing in January 2020. Blood samples, collected for a previous study from a convenience sample of Huntaway dogs (n = 115) from rural regions throughout the North and South Islands of New Zealand between August 2018 and December 2020, were also tested. DNA was extracted and quantitative PCR was used to detect the T. orientalis Ikeda major piroplasm surface protein (MPSP) gene. A standard curve of five serial 10-fold dilutions of a plasmid carrying a fragment of the T. orientalis MPSP gene was used to quantify the number of T. orientalis organisms in the samples. MPSP amplicons obtained by end-point PCR on positive samples were isolated and subjected to DNA sequencing. The resulting sequences were compared to previously published T. orientalis sequences. RESULTS: There were 6/57 (10%) samples positive for T. orientalis Ikeda from the deer and no samples positive for T. orientalis Ikeda from the working dogs or horses. The mean infection intensity for the six PCR-positive deer was 5.1 (min 2.2, max 12.4) T. orientalis Ikeda organisms/µL. CONCLUSIONS AND CLINICAL RELEVANCE: Red deer can potentially sustain low infection intensities of T. orientalis Ikeda and could act as reservoirs of infected ticks. Further studies are needed to determine whether naïve ticks feeding on infected red deer can themselves become infected. ABBREVIATIONS: Cq: Quantification cycle; LOQ: Limits of quantification; MPSP: Major piroplasm surface protein; qPCR: Quantitative polymerase chain reaction.
dc.format.pagination1-5
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/39222954
dc.identifier.citationLawrence KE, Gedye K, Carvalho L, Wang B, Fermin LM, Pomroy WE. (2024). Theileria orientalis Ikeda infection detected in red deer but not dogs or horses in New Zealand.. N Z Vet J. (pp. 1-5).
dc.identifier.doi10.1080/00480169.2024.2393679
dc.identifier.eissn1176-0710
dc.identifier.elements-typejournal-article
dc.identifier.issn0048-0169
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71501
dc.languageeng
dc.relation.isPartOfN Z Vet J
dc.rights(c) The author/sen
dc.rights.licenseCC BY-NC-NDen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectNew Zealand
dc.subjectTheileria orientalis Ikeda
dc.subjectTheileriosis
dc.subjectcanine
dc.subjectequine
dc.subjectred deer
dc.titleTheileria orientalis Ikeda infection detected in red deer but not dogs or horses in New Zealand.
dc.typeJournal article
pubs.elements-id491403
pubs.organisational-groupCollege of Sciences
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