Dissecting the relationship between plasma and tissue metabolome in a cohort of women with obesity: Analysis of subcutaneous and visceral adipose, muscle, and liver

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dc.citation.issue7
dc.citation.volume36
dc.contributor.authorWu ZE
dc.contributor.authorKruger MC
dc.contributor.authorCooper GJS
dc.contributor.authorSequeira IR
dc.contributor.authorMcGill A-T
dc.contributor.authorPoppitt SD
dc.contributor.authorFraser K
dc.coverage.spatialUnited States
dc.date.accessioned2023-07-18T02:13:41Z
dc.date.accessioned2023-09-04T01:40:48Z
dc.date.available2022-06-15
dc.date.available2023-07-18T02:13:41Z
dc.date.available2023-09-04T01:40:48Z
dc.date.issued2022-07
dc.date.updated2023-07-17T00:19:54Z
dc.description© 2022 The Authorsen_US
dc.description.abstractUntargeted metabolomics of blood samples has become widely applied to study metabolic alterations underpinning disease and to identify biomarkers. However, understanding the relevance of a blood metabolite marker can be challenging if it is unknown whether it reflects the concentration in relevant tissues. To explore this field, metabolomic and lipidomic profiles of plasma, four sites of adipose tissues (ATs) from peripheral or central depot, two sites of muscle tissue, and liver tissue from a group of nondiabetic women with obesity who were scheduled to undergo bariatric surgery (n = 21) or other upper GI surgery (n = 5), were measured by liquid chromatography coupled with mass spectrometry. Relationships between plasma and tissue profiles were examined using Pearson correlation analysis subject to Benjamini-Hochberg correction. Plasma metabolites and lipids showed the highest number of significantly positive correlations with their corresponding concentrations in liver tissue, including lipid species of ceramide, mono- and di-hexosylceramide, sphingomyelin, phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysophosphatidylethanolamine, dimethyl phosphatidylethanolamine, ether-linked PC, ether-linked PE, free fatty acid, cholesteryl ester, diacylglycerol and triacylglycerol, and polar metabolites linked to several metabolic functions and gut microbial metabolism. Plasma also showed significantly positive correlations with muscle for several phospholipid species and polar metabolites linked to metabolic functions and gut microbial metabolism, and with AT for several triacylglycerol species. In conclusion, plasma metabolomic and lipidomic profiles were reflective more of the liver profile than any of the muscle or AT sites examined in the present study. Our findings highlighted the importance of taking into consideration the metabolomic relationship of various tissues with plasma when postulating plasma metabolites marker to underlying mechanisms occurring in a specific tissue.
dc.description.confidentialfalse
dc.edition.editionJuly 2022
dc.format.extente22371-
dc.identifiere22371
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/35704337
dc.identifier.citationWu ZE, Kruger MC, Cooper GJS, Sequeira IR, McGill A-T, Poppitt SD, Fraser K. (2022). Dissecting the relationship between plasma and tissue metabolome in a cohort of women with obesity: Analysis of subcutaneous and visceral adipose, muscle, and liver.. FASEB J. 36. 7. (pp. e22371-).
dc.identifier.doi10.1096/fj.202101812R
dc.identifier.eissn1530-6860
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0892-6638
dc.identifier.urihttp://hdl.handle.net/10179/19880
dc.languageeng
dc.publisherWiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology
dc.publisher.urihttps://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202101812R
dc.relation.isPartOfFASEB J
dc.rightsCC BY 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectbiomarkers
dc.subjectclinical metabolomics
dc.subjectliquid chromatography-mass spectrometry
dc.subjecttissue metabolomics
dc.subjectBiomarkers
dc.subjectEthers
dc.subjectFemale
dc.subjectHumans
dc.subjectLiver
dc.subjectMetabolome
dc.subjectMetabolomics
dc.subjectMuscles
dc.subjectObesity
dc.subjectPhosphatidylcholines
dc.subjectPhosphatidylethanolamines
dc.subjectTriglycerides
dc.titleDissecting the relationship between plasma and tissue metabolome in a cohort of women with obesity: Analysis of subcutaneous and visceral adipose, muscle, and liver
dc.typeJournal article
pubs.elements-id454032
pubs.organisational-groupOther
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