IKVAV-linked cell membrane-spanning peptide treatment induces neuronal reactivation following spinal cord injury
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Date
27/10/2015
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Abstract
Spinal cord regeneration following treatment with a novel membrane-spanning
peptide (MSP) expressing the isoleucine-lysine-valine-alanine-valine (IKVAV) epitope
was assessed in Balb-c mice. After hemilaminectomy and compression injury, mice were
treated with IKVAV, IKVAV-MSP, peptide or vehicle control. Functional improvement
was assessed using modified Basso, Beattie, and Bresnahan Scale (mBBB) and spinal
cord segments were studied histologically 28 days after injury. IKVAV-MSP group scores
increased significantly compared with control groups after 4 weeks of observation
(p < 0.05). The number of protoplasmic astrocytes, neurons and muscle bundle size in
the IKVAV-MSP mice were significantly increased (p < 0.001; p < 0.05 and p < 0.007;
respectively). This study demonstrates that it is possible to promote functional recovery
after SCI using bioactive IKVAV presenting cell membrane-spanning peptides.
Lay abstract: A new potential treatment for spinal cord injury was tested in mice.
Mice were treated with our membrane-spanning peptide with the IKVAV motif
(IKVAV-MSP) or various control treatments. Functional improvement was assessed
daily and spinal cord segments were evaluated after 4 weeks. IKVAV-MSP treatment
significantly restored function compared with control groups, numbers of health
nerve cells within the spinal cord were increased and muscles appeared healthier. This
study demonstrates that it is possible to promote functional recovery after SCI using
bioactive IKVAV presenting cell membrane-spanning peptides.
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Keywords
spinal cord injury, regeneration, IKVAV, regeneration, membrane-spanning peptide
Citation
Future Science OA, 2015, 1 (4), pp. 1 - 10