Unambiguous identification of Ancylostoma caninum and Uncinaria stenocephala in Australian and New Zealand dogs from faecal samples.
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Date
2023-10-01
Open Access Location
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley and Sons, Inc.
Rights
(c) 2023 The Author/s
CC BY 4.0
CC BY 4.0
Abstract
Hookworms (Ancylostomatidae) are well-known parasites in dogs due to their health impacts and zoonotic potential. While faecal analysis is the traditional method for detection, improvements in husbandry and deworming have decreased their prevalence in urban owned dogs. Drug resistance in Ancylostoma caninum is becoming a discussion point in small animal practices across the region. This study aimed to identify hookworm species present in Australian and New Zealand dogs using molecular techniques. The ITS-2 and isotype-1 β-tubulin assays were used to identify and quantify hookworm species. Results showed absence of coinfection in Australian samples from Greater Sydney region belonging either to A. caninum or Uncinaria stenocephala, while New Zealand samples were a mixture of A. caninum and U. stenocephala. The amplified isotype-1 β-tubulin sequences exhibited susceptibility to benzimidazole drugs. Rare mutations were identified in A. caninum and U. stenocephala sequences, representing a small percentage of reads. This study highlights the importance of molecular techniques in accurately identifying and quantifying hookworm species in dog populations.
Description
Keywords
ITS-2, drug resistance, hookworms, nemabiome, tubulin, Dogs, Animals, Ancylostoma, Ancylostomatoidea, New Zealand, Tubulin, Dog Diseases, Australia, Hookworm Infections, Feces
Citation
Stocker T, Scott I, Šlapeta J. (2023). Unambiguous identification of Ancylostoma caninum and Uncinaria stenocephala in Australian and New Zealand dogs from faecal samples.. Aust Vet J. 101. 10. (pp. 373-376).