Gelatin and Collagen from Sheepskin.

Simple item page
dc.citation.issue11
dc.citation.volume16
dc.contributor.authorMatinong AME
dc.contributor.authorPickering KL
dc.contributor.authorWaterland MR
dc.contributor.authorChisti Y
dc.contributor.authorHaverkamp RG
dc.contributor.editorYu L
dc.contributor.editorPopa M
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-07-12T02:34:44Z
dc.date.available2024-07-12T02:34:44Z
dc.date.issued2024-05-31
dc.description.abstractAbattoirs dispose of sheepskins as solid waste due to low price and poor demand for sheepskin leather. In principle, as an alternative to being disposed of in landfill, sheepskins can serve as a source of the protein collagen or the hydrolysis product, gelatin. In this research, sheepskins collected from abattoirs were used as a source of collagen. Three extraction methods were compared: acid extraction, acid with enzymes, and alkali extraction. The extracted material was characterized using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR), small angle X-ray scattering (SAXS), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The collagen and gelatin extraction yield ranged from 3.1% to 4.8% with the product purity determined by hydroxyproline, ranging from 7.8% for the alkali process to 59% and 68% for the acid and acid-enzyme processes. SDS PAGE showed that the acid process produced fragments with molecular weights in the range 100 to >250 kDa, while acid-enzyme resulted in smaller fragments, below 30 kDa. The FTIR region of the amide I band at 1800-1550 cm-1, which was used as an indicator of the collagen and gelatin content, showed that the gelatin dominated in the acid extracts, and the alkaline extract contained a large portion of keratin. SAXS was found to be a sensitive method for showing the presence of intact collagen fibrils in materials from all of the extraction methods, albeit at low concentrations. Herein, sheepskin is shown to be a useful source for collagen-gelatin material of varying molecular weights.
dc.description.confidentialfalse
dc.edition.editionJune-1 2024
dc.format.pagination1563-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/38891509
dc.identifier.citationMatinong AME, Pickering KL, Waterland MR, Chisti Y, Haverkamp RG. (2024). Gelatin and Collagen from Sheepskin.. Polymers (Basel). 16. 11. (pp. 1563-).
dc.identifier.doi10.3390/polym16111563
dc.identifier.eissn2073-4360
dc.identifier.elements-typejournal-article
dc.identifier.issn2073-4360
dc.identifier.number1563
dc.identifier.piipolym16111563
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70166
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2073-4360/16/11/1563
dc.relation.isPartOfPolymers (Basel)
dc.rights(c) 2024 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectcollagen
dc.subjectextraction
dc.subjectgelatin
dc.subjectsheepskin
dc.titleGelatin and Collagen from Sheepskin.
dc.typeJournal article
pubs.elements-id489387
pubs.organisational-groupCollege of Health
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