Phenotypic characterization and genome analysis of a novel Salmonella Typhimurium phage having unique tail fiber genes.

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dc.citation.issue1
dc.citation.volume12
dc.contributor.authorSattar S
dc.contributor.authorUllah I
dc.contributor.authorKhanum S
dc.contributor.authorBailie M
dc.contributor.authorShamsi B
dc.contributor.authorAhmed I
dc.contributor.authorShah STA
dc.contributor.authorJaved S
dc.contributor.authorGhafoor A
dc.contributor.authorPervaiz A
dc.contributor.authorSohail F
dc.contributor.authorShah NA
dc.contributor.authorImdad K
dc.contributor.authorBostan N
dc.contributor.authorAltermann E
dc.coverage.spatialEngland
dc.date.accessioned2024-09-25T03:12:59Z
dc.date.available2024-09-25T03:12:59Z
dc.date.issued2022-04-06
dc.description.abstractSalmonella enterica serovar Typhimurium is a foodborne pathogen causing occasional outbreaks of enteric infections in humans. Salmonella has one of the largest pools of temperate phages in its genome that possess evolutionary significance for pathogen. In this study, we characterized a novel temperate phage Salmonella phage BIS20 (BIS20) with unique tail fiber genes. It belongs to the subfamily Peduovirinae genus Eganvirus and infects Salmonella Typhimurium strain (SE-BS17; Acc. NO MZ503545) of poultry origin. Phage BIS20 was viable only at biological pH and temperature ranges (pH7 and 37 °C). Despite being temperate BIS20 significantly slowed down the growth of host strain for 24 h as compared to control (P < 0.009). Phage BIS20 features 29,477-base pair (bp) linear DNA genome with 53% GC content and encodes for 37 putative ORFs. These ORFs have mosaic arrangement as indicated by its ORF similarity to various phages and prophages in NCBI. Genome analysis indicates its similarity to Salmonella enterica serovar Senftenberg prophage (SEStP) sequence (Nucleotide similarity 87.7%) and Escherichia virus 186 (~ 82.4% nucleotide similarity). Capsid genes were conserved however those associated with tail fiber formation and assembly were unique to all members of genus Eganvirus. We found strong evidence of recombination hotspot in tail fiber gene. Our study identifies BIS20 as a new species of genus Eganvirus temperate phages as its maximum nucleotide similarity is 82.4% with any phage in NCBI. Our findings may contribute to understanding of origin of new temperate phages.
dc.description.confidentialfalse
dc.edition.edition2022
dc.format.pagination5732-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35388062
dc.identifier.citationSattar S, Ullah I, Khanum S, Bailie M, Shamsi B, Ahmed I, Shah STA, Javed S, Ghafoor A, Pervaiz A, Sohail F, Shah NA, Imdad K, Bostan N, Altermann E. (2022). Phenotypic characterization and genome analysis of a novel Salmonella Typhimurium phage having unique tail fiber genes.. Sci Rep. 12. 1. (pp. 5732-).
dc.identifier.doi10.1038/s41598-022-09733-5
dc.identifier.eissn2045-2322
dc.identifier.elements-typejournal-article
dc.identifier.issn2045-2322
dc.identifier.number5732
dc.identifier.pii10.1038/s41598-022-09733-5
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71520
dc.languageeng
dc.publisherSpringer Nature Limited
dc.publisher.urihttps://www.nature.com/articles/s41598-022-09733-5
dc.relation.isPartOfSci Rep
dc.rights(c) 2022 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBacteriophages
dc.subjectGenome, Viral
dc.subjectHumans
dc.subjectMyoviridae
dc.subjectNucleotides
dc.subjectProphages
dc.subjectSalmonella
dc.subjectSalmonella Phages
dc.subjectSalmonella typhimurium
dc.titlePhenotypic characterization and genome analysis of a novel Salmonella Typhimurium phage having unique tail fiber genes.
dc.typeJournal article
pubs.elements-id486127
pubs.organisational-groupOther
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