Intragastric restructuring dictates the digestive kinetics of heat-set milk protein gels of contrasting textures
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Date
2024-11
Open Access Location
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Rights
(c) The author/s
CC BY
CC BY
Abstract
The gelation of milk proteins can be achieved by various means, enabling the development of diverse products. In this study, heat-set milk protein gels (15 % protein) of diverse textures were made by pH modulation and two gels were selected for dynamic in vitro gastric digestion: a spoonable soft gel (SG, pH 6.55' G' of ∼100 Pa) and a sliceable firm gel (FG, pH 5.65; G' of ∼7000 Pa). The two gels displayed markedly different structural changes and digestion kinetics during gastric digestion. The SG underwent substantial structural compaction during the first 120 min of gastric digestion into a denser and firmer gastric chyme (26.3 % crude protein, G* of ∼8500 Pa) than the chyme of the FG (15.7 % crude protein, G* of ∼3000 Pa). These contrasting intragastric structural changes of the gels reversed their original textural differences, which led to slower digestion and gastric emptying of proteins from the SG compared with the FG. The different intragastric pH profiles during the digestion of the two gels likely played a key role by modulating the proteolytic activity and specificity (to κ-casein) of pepsin. Preferential early cleavage of κ-casein in SG stimulated coagulation and compaction of solid chyme, whereas rapid hydrolysis of αS- and β-caseins in the FG weakened coagulation. This study provided new insights into controlling the structural development of dairy-based foods during gastric digestion and modulating digestion kinetics.
Description
Keywords
Food structure, Gastric restructuring, In vitro digestion, Milk protein gels, Protein digestion, Gels, Digestion, Hydrogen-Ion Concentration, Kinetics, Milk Proteins, Hot Temperature, Gastric Emptying, Caseins, Pepsin A, Animals, Food Handling, Proteolysis
Citation
Li S, Mungure T, Ye A, Loveday SM, Ellis A, Weeks M, Singh H. (2024). Intragastric restructuring dictates the digestive kinetics of heat-set milk protein gels of contrasting textures.. Food Res Int. 195. (pp. 114944-).