RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue

Graña-Baumgartner A; Dukkipati VSR; Kenyon PR; Blair HT; Lopez-Villalobos N; Gedye K; Biggs PJ

RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue

dc.citation.issue	10
dc.citation.volume	12
dc.contributor.author	Graña-Baumgartner A
dc.contributor.author	Dukkipati VSR
dc.contributor.author	Kenyon PR
dc.contributor.author	Blair HT
dc.contributor.author	Lopez-Villalobos N
dc.contributor.author	Gedye K
dc.contributor.author	Biggs PJ
dc.contributor.editor	Kadegowda AKG
dc.contributor.editor	Bionaz M
dc.coverage.spatial	Switzerland
dc.date.accessioned	2024-01-04T01:41:21Z
dc.date.accessioned	2024-07-25T06:46:33Z
dc.date.available	2022-10-20
dc.date.available	2024-01-04T01:41:21Z
dc.date.available	2024-07-25T06:46:33Z
dc.date.issued	2022-10-20
dc.description.abstract	During the early postnatal period, lambs have the ability to thermoregulate body temperature via non-shivering thermogenesis through brown adipose tissue (BAT), which soon after birth begins to transform into white adipose tissue. An RNA seq approach was used to characterize the transcriptome of BAT and thyroid tissue in newborn lambs exposed to cold conditions. Fifteen newborn Romney lambs were selected and divided into three groups: group 1 (n = 3) was a control, and groups 2 and 3 (n = 6 each) were kept indoors for two days at an ambient temperature (20-22 °C) or at a cold temperature (4 °C), respectively. Sequencing was performed using a paired-end strategy through the BGISEQ-500 platform, followed by the identification of differentially expressed genes using DESeq2 and an enrichment analysis by g:Profiler. This study provides an in-depth expression network of the main characters involved in the thermogenesis and fat-whitening mechanisms that take place in the newborn lamb. Data revealed no significant differential expression of key thermogenic factors such as uncoupling protein 1, suggesting that the heat production peak under cold exposure might occur so rapidly and in such an immediate way that it may seem undetectable in BAT by day three of life. Moreover, these changes in expression might indicate the start of the whitening process of the adipose tissue, concluding the non-shivering thermogenesis period.
dc.description.confidential	false
dc.edition.edition	October 2022
dc.format.pagination	996-
dc.identifier.author-url	https://www.ncbi.nlm.nih.gov/pubmed/36295898
dc.identifier.citation	Graña-Baumgartner A, Dukkipati VSR, Kenyon PR, Blair HT, López-Villalobos N, Gedye K, Biggs PJ. (2022). RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue.. Metabolites. 12. 10. (pp. 996-).
dc.identifier.doi	10.3390/metabo12100996
dc.identifier.eissn	2218-1989
dc.identifier.elements-type	journal-article
dc.identifier.issn	2218-1989
dc.identifier.number	996
dc.identifier.pii	metabo12100996
dc.identifier.uri	https://mro.massey.ac.nz/handle/10179/70846
dc.language	eng
dc.publisher	MDPI (Basel, Switzerland)
dc.publisher.uri	https://www.mdpi.com/2218-1989/12/10/996
dc.relation.isPartOf	Metabolites
dc.rights	(c) The author/s	en
dc.rights.license	CC BY 4.0	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject	BAT
dc.subject	RNAseq
dc.subject	fat whitening
dc.subject	lambs
dc.subject	thermogenesis
dc.title	RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue
dc.type	Journal article
pubs.elements-id	457542
pubs.organisational-group	Other