Culture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers

Simple item page
dc.citation.issue2
dc.citation.volume12
dc.contributor.authorBarnett AM
dc.contributor.authorMullaney JA
dc.contributor.authorMcNabb WC
dc.contributor.authorRoy NC
dc.coverage.spatialUnited States
dc.date.accessioned2024-11-06T00:24:17Z
dc.date.available2024-11-06T00:24:17Z
dc.date.issued2024-04-02
dc.description.abstractIntestinal organoid technology has revolutionized our approach to in vitro cell culture due in part to their three-dimensional structures being more like the native tissue from which they were derived with respect to cellular composition and architecture. For this reason, organoids are becoming the new gold standard for undertaking intestinal epithelial cell research. Unfortunately, their otherwise advantageous three-dimensional geometry prevents easy access to the apical epithelium, which is a major limitation when studying interactions between dietary or microbial components and host tissues. To overcome this problem, we developed porcine colonoid-derived monolayers cultured on both permeable Transwell inserts and tissue culture treated polystyrene plates. We found that seeding density and culture format altered the expression of genes encoding markers of specific cell types (stem cells, colonocytes, goblets, and enteroendocrine cells), and barrier maturation (tight junctions). Additionally, we found that changes to the formulation of the culture medium altered the cellular composition of colonoids and of monolayers derived from them, resulting in cultures with an increasingly differentiated phenotype that was similar to that of their tissue of origin.
dc.description.confidentialfalse
dc.format.pagination2222632-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37340938
dc.identifier.citationBarnett AM, Mullaney JA, McNabb WC, Roy NC. (2024). Culture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers.. Tissue Barriers. 12. 2. (pp. 2222632-).
dc.identifier.doi10.1080/21688370.2023.2222632
dc.identifier.eissn2168-8370
dc.identifier.elements-typejournal-article
dc.identifier.issn2168-8362
dc.identifier.number2222632
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71921
dc.languageeng
dc.publisherTaylor and Francis Group
dc.publisher.urihttps://www.tandfonline.com/doi/full/10.1080/21688370.2023.2222632
dc.relation.isPartOfTissue Barriers
dc.rights(c) 2023 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBarrier integrity
dc.subjectPorcine colonoids
dc.subjectcolonoid-derived monolayers
dc.subjectdifferentiation
dc.subjectmedium formulation
dc.subjectstem cells
dc.subjectAnimals
dc.subjectSwine
dc.subjectCulture Media
dc.subjectColon
dc.subjectOrganoids
dc.subjectIntestinal Mucosa
dc.subjectCell Culture Techniques
dc.titleCulture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers
dc.typeJournal article
pubs.elements-id462382
pubs.organisational-groupOther
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