Genome sequence of the entomopathogenic Serratia entomophila isolate 626 and characterisation of the species specific itaconate degradation pathway

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dc.citation.issue1
dc.citation.volume23
dc.contributor.authorVaughan AL
dc.contributor.authorAltermann E
dc.contributor.authorGlare TR
dc.contributor.authorHurst MRH
dc.coverage.spatialEngland
dc.date.accessioned2024-07-23T23:50:09Z
dc.date.available2024-07-23T23:50:09Z
dc.date.issued2022-12
dc.description.abstractBACKGROUND: Isolates of Serratia entomophila and S. proteamaculans (Yersiniaceae) cause disease specific to the endemic New Zealand pasture pest, Costelytra giveni (Coleoptera: Scarabaeidae). Previous genomic profiling has shown that S. entomophila isolates appear to have conserved genomes and, where present, conserved plasmids. In the absence of C. giveni larvae, S. entomophila prevalence reduces in the soil over time, suggesting that S. entomophila has formed a host-specific relationship with C. giveni. To help define potential genetic mechanisms driving retention of the chronic disease of S. entomophila, the genome of the isolate 626 was sequenced, enabling the identification of unique chromosomal properties, and defining the gain/loss of accessory virulence factors relevant to pathogenicity to C. giveni larvae. RESULTS: We report the complete sequence of S. entomophila isolate 626, a causal agent of amber disease in C. giveni larvae. The genome of S. entomophila 626 is 5,046,461 bp, with 59.1% G + C content and encoding 4,695 predicted CDS. Comparative analysis with five previously sequenced Serratia species, S. proteamaculans 336X, S. marcescens Db11, S. nematodiphila DH-S01, S. grimesii BXF1, and S. ficaria NBRC 102596, revealed a core of 1,165 genes shared. Further comparisons between S. entomophila 626 and S. proteamaculans 336X revealed fewer predicted phage-like regions and genomic islands in 626, suggesting less horizontally acquired genetic material. Genomic analyses revealed the presence of a four-gene itaconate operon, sharing a similar gene order as the Yersinia pestis ripABC complex. Assessment of a constructed 626::RipC mutant revealed that the operon confer a possible metabolic advantage to S. entomophila in the initial stages of C. giveni infection. CONCLUSIONS: Evidence is presented where, relative to S. proteamaculans 336X, S. entomophila 626 encodes fewer genomic islands and phages, alluding to limited horizontal gene transfer in S. entomophila. Bioassay assessments of a S. entomophila-mutant with a targeted mutation of the itaconate degradation region unique to this species, found the mutant to have a reduced capacity to replicate post challenge of the C. giveni larval host, implicating the itaconate operon in establishment within the host.
dc.description.confidentialfalse
dc.edition.editionDecember 2022
dc.format.pagination728-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36303123
dc.identifier.citationVaughan AL, Altermann E, Glare TR, Hurst MRH. (2022). Genome sequence of the entomopathogenic Serratia entomophila isolate 626 and characterisation of the species specific itaconate degradation pathway.. BMC Genomics. 23. 1. (pp. 728-).
dc.identifier.doi10.1186/s12864-022-08938-2
dc.identifier.eissn1471-2164
dc.identifier.elements-typejournal-article
dc.identifier.issn1471-2164
dc.identifier.number728
dc.identifier.pii10.1186/s12864-022-08938-2
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70297
dc.languageeng
dc.publisherBioMed Central Ltd
dc.publisher.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-022-08938-2
dc.relation.isPartOfBMC Genomics
dc.rights(c) 2022 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectChromosome
dc.subjectEntomopathogen
dc.subjectGenome
dc.subjectHorizontal gene transfer
dc.subjectItaconate
dc.subjectVirulence
dc.subjectAnimals
dc.subjectSerratia
dc.subjectVirulence
dc.subjectPlasmids
dc.subjectColeoptera
dc.subjectLarva
dc.subjectSerratia marcescens
dc.titleGenome sequence of the entomopathogenic Serratia entomophila isolate 626 and characterisation of the species specific itaconate degradation pathway
dc.typeJournal article
pubs.elements-id486124
pubs.organisational-groupOther
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