Classification of Bartonella strains associated with straw-colored fruit bats (Eidolon helvum) across Africa using a multi-locus sequence typing platform.

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dc.citation.issue1
dc.citation.volume9
dc.contributor.authorBai Y
dc.contributor.authorHayman DTS
dc.contributor.authorMcKee CD
dc.contributor.authorKosoy MY
dc.date.available2015-01-01
dc.date.available2014-12-13
dc.date.issued2015-01
dc.description.abstractBartonellae are facultative intracellular bacteria and are highly adapted to their mammalian host cell niches. Straw-colored fruit bats (Eidolon helvum) are commonly infected with several bartonella strains. To elucidate the genetic diversity of these bartonella strains, we analyzed 79 bartonella isolates from straw-colored fruit bats in seven countries across Africa (Cameroon, Annobon island of Equatorial Guinea, Ghana, Kenya, Nigeria, Tanzania, and Uganda) using a multi-locus sequencing typing (MLST) approach based on nucleotide sequences of eight loci (ftsZ, gltA, nuoG, ribC, rpoB, ssrA, ITS, and 16S rRNA). The analysis of each locus but ribC demonstrated clustering of the isolates into six genogroups (E1 - E5 and Ew), while ribC was absent in the isolates belonging to the genogroup Ew. In general, grouping of all isolates by each locus was mutually supportive; however, nuoG, gltA, and rpoB showed some incongruity with other loci in several strains, suggesting a possibility of recombination events, which were confirmed by network analyses and recombination/mutation rate ratio (r/m) estimations. The MLST scheme revealed 45 unique sequence types (ST1 - 45) among the analyzed bartonella isolates. Phylogenetic analysis of concatenated sequences supported the discrimination of six phylogenetic lineages (E1 - E5 and Ew) corresponding to separate and unique Bartonella species. One of the defined lineages, Ew, consisted of only two STs (ST1 and ST2), and comprised more than one-quarter of the analyzed isolates, while other lineages contained higher numbers of STs with a smaller number of isolates belonging to each lineage. The low number of allelic polymorphisms of isolates belonging to Ew suggests a more recent origin for this species. Our findings suggest that at least six Bartonella species are associated with straw-colored fruit bats, and that distinct STs can be found across the distribution of this bat species, including in populations of bats which are genetically distinct.
dc.description.publication-statusPublished
dc.format.extent? - ? (16)
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000349318100055&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN e0003478
dc.identifier.citationPLOS NEGLECTED TROPICAL DISEASES, 2015, 9 (1), pp. ? - ? (16)
dc.identifier.doi10.1371/journal.pntd.0003478
dc.identifier.elements-id223706
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1935-2735
dc.identifier.urihttps://hdl.handle.net/10179/7464
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.isPartOfPLOS NEGLECTED TROPICAL DISEASES
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectInfectious Diseases
dc.subjectParasitology
dc.subjectTropical Medicine
dc.subjectCAT-SCRATCH DISEASE
dc.subjectSP-NOV
dc.subjectHENSELAE
dc.subjectSPP.
dc.subjectRECOMBINATION
dc.subjectVINSONII
dc.subjectPATHOGEN
dc.subjectREVEALS
dc.subjectRODENTS
dc.subjectPATIENT
dc.subject.anzsrc06 Biological Sciences
dc.subject.anzsrc11 Medical and Health Sciences
dc.titleClassification of Bartonella strains associated with straw-colored fruit bats (Eidolon helvum) across Africa using a multi-locus sequence typing platform.
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Veterinary Science
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