Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change

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dc.citation.issue1
dc.citation.volume22
dc.contributor.authorLaible G
dc.contributor.authorCole S-A
dc.contributor.authorBrophy B
dc.contributor.authorWei J
dc.contributor.authorLeath S
dc.contributor.authorJivanji S
dc.contributor.authorLittlejohn MD
dc.contributor.authorWells DN
dc.coverage.spatialEngland
dc.date.accessioned2024-07-03T23:20:53Z
dc.date.available2024-07-03T23:20:53Z
dc.date.issued2021-11-26
dc.description.abstractBACKGROUND: High-producing Holstein Friesian dairy cattle have a characteristic black and white coat, often with large proportions of black. Compared to a light coat color, black absorbs more solar radiation which is a contributing factor to heat stress in cattle. To better adapt dairy cattle to rapidly warming climates, we aimed to lighten their coat color by genome editing. RESULTS: Using gRNA/Cas9-mediated editing, we introduced a three bp deletion in the pre-melanosomal protein 17 gene (PMEL) proposed as causative variant for the semi-dominant color dilution phenotype observed in Galloway and Highland cattle. Calves generated from cells with homozygous edits revealed a strong color dilution effect. Instead of the characteristic black and white markings of control calves generated from unedited cells, the edited calves displayed a novel grey and white coat pattern. CONCLUSION: This, for the first time, verified the causative nature of the PMEL mutation for diluting the black coat color in cattle. Although only one of the calves was healthy at birth and later succumbed to a naval infection, the study showed the feasibility of generating such edited animals with the possibility to dissect the effects of the introgressed edit and other interfering allelic variants that might exist in individual cattle and accurately determine the impact of only the three bp change.
dc.description.confidentialfalse
dc.edition.edition2021
dc.format.pagination856-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/34836496
dc.identifier.citationLaible G, Cole S-A, Brophy B, Wei J, Leath S, Jivanji S, Littlejohn MD, Wells DN. (2021). Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change.. BMC Genomics. 22. 1. (pp. 856-).
dc.identifier.doi10.1186/s12864-021-08175-z
dc.identifier.eissn1471-2164
dc.identifier.elements-typejournal-article
dc.identifier.issn1471-2164
dc.identifier.numberARTN 856
dc.identifier.pii10.1186/s12864-021-08175-z
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70075
dc.languageeng
dc.publisherBioMed Central Ltd
dc.publisher.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-08175-z
dc.relation.isPartOfBMC Genomics
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCas9
dc.subjectCattle
dc.subjectCoat color
dc.subjectGenome editing
dc.subjectHomology-directed repair, PMEL
dc.subjectP.Leu18del
dc.subjectSignal peptide
dc.subjectSomatic cell nuclear transfer
dc.subjectWhite spotting
dc.subjectAnimals
dc.subjectCattle
dc.subjectClimate Change
dc.subjectGene Editing
dc.subjectHeat Stress Disorders
dc.subjectHeat-Shock Response
dc.subjectPhenotype
dc.titleHolstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change
dc.typeJournal article
pubs.elements-id449874
pubs.organisational-groupOther
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