Validation of an Indirect Immunofluorescence Assay and Commercial Q Fever Enzyme-Linked Immunosorbent Assay for Use in Macropods

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dc.citation.issue7
dc.citation.volume60
dc.contributor.authorTolpinrud A
dc.contributor.authorStenos J
dc.contributor.authorChaber A-L
dc.contributor.authorDevlin JM
dc.contributor.authorHerbert C
dc.contributor.authorPas A
dc.contributor.authorDunowska M
dc.contributor.authorStevenson MA
dc.contributor.authorFirestone SM
dc.contributor.editorBarrs, VR
dc.coverage.spatialUnited States
dc.date.accessioned2024-01-19T02:01:18Z
dc.date.accessioned2024-07-25T06:43:09Z
dc.date.available2022-06-02
dc.date.available2024-01-19T02:01:18Z
dc.date.available2024-07-25T06:43:09Z
dc.date.issued2022-07
dc.description.abstractKangaroos are considered to be an important reservoir of Q fever in Australia, although there is limited knowledge on the true prevalence and distribution of coxiellosis in Australian macropod populations. Serological tests serve as useful surveillance tools, but formal test validation is needed to be able to estimate true seroprevalence rates, and few tests have been validated to screen wildlife species for Q fever. In this study, we modified and optimized a phase-specific indirect immunofluorescence assay (IFA) for the detection of IgG antibodies against Coxiella burnetii in macropod sera. The assay was validated against the commercially available ID Screen Q fever indirect multispecies enzyme-linked immunosorbent assay (ELISA) kit (IDVet, Grabels, France) to estimate the diagnostic sensitivity and specificity of each assay, using Bayesian latent class analysis. A direct comparison of the two tests was performed by testing 303 serum samples from 10 macropod populations from the east coast of Australia and New Zealand. The analysis indicated that the IFA had relatively high diagnostic sensitivity (97.6% [95% credible interval [CrI], 88.0 to 99.9]) and diagnostic specificity (98.5% [95% CrI, 94.4 to 99.9]). In comparison, the ELISA had relatively poor diagnostic sensitivity (42.1% [95% CrI, 33.7 to 50.8]) and similar diagnostic specificity (99.2% [95% CrI, 96.4 to 100]) using the cutoff values recommended by the manufacturer. The estimated true seroprevalence of C. burnetii exposure in the macropod populations included in this study ranged from 0% in New Zealand and Victoria, Australia, up to 94.2% in one population from New South Wales, Australia.
dc.description.confidentialfalse
dc.edition.editionJuly 2022
dc.format.paginatione0023622-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35652310
dc.identifier.citationTolpinrud A, Stenos J, Chaber A-L, Devlin JM, Herbert C, Pas A, Dunowska M, Stevenson MA, Firestone SM. (2022). Validation of an Indirect Immunofluorescence Assay and Commercial Q Fever Enzyme-Linked Immunosorbent Assay for Use in Macropods.. J Clin Microbiol. 60. 7. (pp. e0023622-).
dc.identifier.doi10.1128/jcm.00236-22
dc.identifier.eissn1098-660X
dc.identifier.elements-typejournal-article
dc.identifier.issn0095-1137
dc.identifier.numbere0023622
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70742
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.publisher.urihttps://journals.asm.org/doi/10.1128/jcm.00236-22
dc.relation.isPartOfJ Clin Microbiol
dc.rights(c) 2022 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBayesian latent class models
dc.subjectCoxiella burnetii
dc.subjectELISA
dc.subjectQ fever
dc.subjectenzyme-linked immunosorbent assay
dc.subjectimmunofluorescence assay
dc.subjectmacropods
dc.subjectsensitivity
dc.subjectspecificity
dc.subjecttest validation
dc.subjectAntibodies, Bacterial
dc.subjectBayes Theorem
dc.subjectCoxiella burnetii
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFluorescent Antibody Technique, Indirect
dc.subjectHumans
dc.subjectQ Fever
dc.subjectSeroepidemiologic Studies
dc.subjectVictoria
dc.titleValidation of an Indirect Immunofluorescence Assay and Commercial Q Fever Enzyme-Linked Immunosorbent Assay for Use in Macropods
dc.typeJournal article
pubs.elements-id453858
pubs.organisational-groupOther
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