Biotransformation of Rutin in In Vitro Porcine Ileal and Colonic Fermentation Models
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Date
2023-08-23
Open Access Location
Journal Title
Journal ISSN
Volume Title
Publisher
American Chemical Society
Rights
(c) 2023 The Author/s
CC BY-NC-ND 4.0
CC BY-NC-ND 4.0
Abstract
Quercetin, a polyphenol antioxidant, is widely distributed in food in the form of glycoside rutin, which is not readily absorbed in the gastrointestinal tract. The microbiota of the colon is known to biotransform rutin, generating quercetin aglycones that can be absorbed. We investigated the role of the ileal and colonic microbiota in rutin biotransformation using established in vitro fermentation models. Overall, a higher rate of rutin biotransformation was observed during colonic fermentation compared with ileal fermentation. The colonic microbiome showed higher potential for rutin conversion to quercetin through an increased abundance of α-rhamnosidase- and β-glucosidase-encoding genes compared to the ileal microbiome. Nonetheless, rutin metabolism occurred rapidly during ileal fermentation (∼20% rutin disappearance after 1 h). The appearance of quercetin varied depending on the ileal inoculum and correlated with an increased abundance of Firmicutes, suggesting that quercetin absorption could be improved via modulation of the ileal microbiota.
Description
Keywords
colon fermentation, fecal microbiome, ileal fermentation, ileal microbiome, polyphenol biotransformation, Swine, Animals, Rutin, Quercetin, Fermentation, Colon, Biotransformation
Citation
Ulluwishewa D, Montoya CA, Mace L, Rettedal EA, Fraser K, McNabb WC, Moughan PJ, Roy NC. (2023). Biotransformation of Rutin in In Vitro Porcine Ileal and Colonic Fermentation Models.. J Agric Food Chem. 71. 33. (pp. 12487-12496).