Widespread occurrence of benzimidazole resistance single nucleotide polymorphisms in the canine hookworm, Ancylostoma caninum, in Australia

Simple item page
dc.citation.issue3-4
dc.citation.volume55
dc.contributor.authorAbdullah S
dc.contributor.authorStocker T
dc.contributor.authorKang H
dc.contributor.authorScott I
dc.contributor.authorHayward D
dc.contributor.authorJaensch S
dc.contributor.authorWard MP
dc.contributor.authorJones MK
dc.contributor.authorKotze AC
dc.contributor.authorŠlapeta J
dc.coverage.spatialEngland
dc.date.accessioned2025-03-27T01:59:35Z
dc.date.available2025-03-27T01:59:35Z
dc.date.issued2025-03
dc.description.abstractCanine hookworm (Ancylostoma caninum), a gastrointestinal nematode of domestic dogs, principally infects the small intestine of dogs and has the potential to cause zoonotic disease. In greyhounds and pet dogs in the USA, A. caninum has been shown to be resistant to multiple anthelmintics. We conducted a molecular survey of benzimidazole resistance in A. caninum from dogs at veterinary diagnostic centers in Australia and New Zealand. First, we implemented an internal transcribed spacer (ITS)-2 rDNA deep amplicon metabarcoding sequencing approach to ascertain the species of hookworms infecting dogs in the region. Then, we evaluated the frequency of the canonical F167Y and Q134H isotype-1 β-tubulin mutations, which confer benzimidazole resistance, using the same sequencing approach. The most detected hookworm species in diagnostic samples was A. caninum (90%; 83/92); the related Northern hookworm (Uncinaria stenocephala) was identified in 11% (10/92) of the diagnostic samples. There was a single sample with coinfection by A. caninum and U. stenocephala. Both isotype-1 β-tubulin mutations were present in A. caninum, 49% and 67% for Q134H and F167Y, respectively. Mutation F167Y in the isotype-1 β-tubulin mutation was recorded in U. stenocephala for the first known time. Canonical benzimidazole resistance codons 198 and 200 mutations were absent. Egg hatch assays performed on a subset of the A. caninum samples showed significant correlation between 50% inhibitory concentration (IC50) to thiabendazole and F167Y, with an increased IC50 for samples with > 75% F167Y mutation. We detected 14% of dogs with > 75% F167Y mutation in A. caninum. Given that these samples were collected from dogs across various regions of Australia, the present study suggests that benzimidazole resistance in A. caninum is widespread. Therefore, to mitigate the risk of resistance selection and further spread, adoption of a risk assessment-based approach to limit unnecessary anthelmintic use should be a key consideration for future parasite control.
dc.description.confidentialfalse
dc.edition.editionMarch 2025
dc.format.pagination173-182
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/39716589
dc.identifier.citationAbdullah S, Stocker T, Kang H, Scott I, Hayward D, Jaensch S, Ward MP, Jones MK, Kotze AC, Šlapeta J. (2025). Widespread occurrence of benzimidazole resistance single nucleotide polymorphisms in the canine hookworm, Ancylostoma caninum, in Australia.. Int J Parasitol. 55. 3-4. (pp. 173-182).
dc.identifier.doi10.1016/j.ijpara.2024.12.001
dc.identifier.eissn1879-0135
dc.identifier.elements-typejournal-article
dc.identifier.issn0020-7519
dc.identifier.piiS0020-7519(24)00227-3
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/72697
dc.languageeng
dc.publisherElsevier Ltd on behalf of the Australian Society for Parasitology Incorporated
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0020751924002273
dc.relation.isPartOfInt J Parasitol
dc.rights(c) 2024 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAncylostoma caninum
dc.subjectAnthelmintics
dc.subjectCanine health
dc.subjectDeep sequencing
dc.subjectGreyhounds
dc.subjectMultiple anthelmintic drug resistant (MADR)
dc.subjectAnimals
dc.subjectDogs
dc.subjectBenzimidazoles
dc.subjectAncylostoma
dc.subjectDog Diseases
dc.subjectAustralia
dc.subjectAncylostomiasis
dc.subjectDrug Resistance
dc.subjectAnthelmintics
dc.subjectPolymorphism, Single Nucleotide
dc.subjectTubulin
dc.subjectDNA, Helminth
dc.subjectNew Zealand
dc.subjectDNA, Ribosomal Spacer
dc.titleWidespread occurrence of benzimidazole resistance single nucleotide polymorphisms in the canine hookworm, Ancylostoma caninum, in Australia
dc.typeJournal article
pubs.elements-id492842
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
492842 PDF.pdf
Size:
794.68 KB
Format:
Adobe Portable Document Format
Description:
Published version.pdf
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
9.22 KB
Format:
Plain Text
Description:
Download
Collections
Journal Articles