Whole genome sequence analysis of ESBL-producing Escherichia coli recovered from New Zealand freshwater sites.

Simple item page
dc.citation.issue10
dc.citation.volume8
dc.contributor.authorBurgess SA
dc.contributor.authorMoinet M
dc.contributor.authorBrightwell G
dc.contributor.authorCookson AL
dc.coverage.spatialEngland
dc.date.available2022-10
dc.date.issued2022-10
dc.descriptionCAUL read and publish agreement 2023
dc.description.abstractExtended-spectrum beta lactamase (ESBL)-producing Escherichia coli are often isolated from humans with urinary tract infections and may display a multidrug-resistant phenotype. These pathogens represent a target for a One Health surveillance approach to investigate transmission between humans, animals and the environment. This study examines the multidrug-resistant phenotype and whole genome sequence data of four ESBL-producing E. coli isolated from freshwater in New Zealand. All four isolates were obtained from a catchment with a mixed urban and pastoral farming land-use. Three isolates were sequence type (ST) 131 (CTX-M-27-positive) and the other ST69 (CTX-M-15-positive); a phylogenetic comparison with other locally isolated strains demonstrated a close relationship with New Zealand clinical isolates. Genes associated with resistance to antifolates, tetracyclines, aminoglycosides and macrolides were identified in all four isolates, together with fluoroquinolone resistance in two isolates. The ST69 isolate harboured the bla CTX-M-15 gene on a IncHI2A plasmid, and two of the three ST131 isolates harboured the bla CTX-M-27 genes on IncF plasmids. The last ST131 isolate harboured bla CTX-M-27 on the chromosome in a unique site between gspC and gspD. These data highlight a probable human origin of the isolates with subsequent transmission from urban centres through wastewater to the wider environment.
dc.description.publication-statusPublished
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/36200854
dc.identifier.citationMicrob Genom, 2022, 8 (10)
dc.identifier.doi10.1099/mgen.0.000893
dc.identifier.eissn2057-5858
dc.identifier.elements-id457251
dc.identifier.harvestedMassey_Dark
dc.identifier.urihttps://hdl.handle.net/10179/18001
dc.languageeng
dc.relation.isPartOfMicrob Genom
dc.subjectESBL
dc.subjectEscherichia coli
dc.subjectantimicrobial resistance
dc.subjectfreshwater
dc.subjectAminoglycosides
dc.subjectAnimals
dc.subjectAnti-Bacterial Agents
dc.subjectEscherichia coli
dc.subjectEscherichia coli Infections
dc.subjectFluoroquinolones
dc.subjectFolic Acid Antagonists
dc.subjectFresh Water
dc.subjectHumans
dc.subjectMacrolides
dc.subjectMicrobial Sensitivity Tests
dc.subjectNew Zealand
dc.subjectPhylogeny
dc.subjectSequence Analysis
dc.subjectTetracyclines
dc.subjectWastewater
dc.subjectbeta-Lactamases
dc.subject.anzsrc0604 Genetics
dc.subject.anzsrc0605 Microbiology
dc.titleWhole genome sequence analysis of ESBL-producing Escherichia coli recovered from New Zealand freshwater sites.
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Veterinary Science
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