Bovine dairy complex lipids improve in vitro measures of small intestinal epithelial barrier integrity

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dc.citation.issue1
dc.citation.volume13
dc.contributor.authorAnderson RC
dc.contributor.authorMacGibbon AKH
dc.contributor.authorHaggarty N
dc.contributor.authorArmstrong KM
dc.contributor.authorRoy NC
dc.contributor.editorBrandner JM
dc.coverage.spatialUnited States
dc.date.accessioned2024-10-31T00:56:05Z
dc.date.available2024-10-31T00:56:05Z
dc.date.issued2018-01-05
dc.description.abstractAppropriate intestinal barrier maturation is essential for absorbing nutrients and preventing pathogens and toxins from entering the body. Compared to breast-fed infants, formula-fed infants are more susceptible to barrier dysfunction-associated illnesses. In infant formula dairy lipids are usually replaced with plant lipids. We hypothesised that dairy complex lipids improve in vitro intestinal epithelial barrier integrity. We tested milkfat high in conjugated linoleic acid, beta serum (SureStart™Lipid100), beta serum concentrate (BSC) and a ganglioside-rich fraction (G600). Using Caco-2 cells as a model of the human small intestinal epithelium, we analysed the effects of the ingredients on trans-epithelial electrical resistance (TEER), mannitol flux, and tight junction protein co-localisation. BSC induced a dose-dependent improvement in TEER across unchallenged cell layers, maintained the co-localisation of tight junction proteins in TNFα-challenged cells with increased permeability, and mitigated the TEER-reducing effects of lipopolysaccharide (LPS). G600 also increased TEER across healthy and LPS-challenged cells, but it did not alter the co-location of tight junction proteins in TNFα-challenged cells. SureStart™Lipid100 had similar TEER-increasing effects to BSC when added at twice the concentration (similar lipid concentration). Ultimately, this research aims to contribute to the development of infant formulas supplemented with dairy complex lipids that support infant intestinal barrier maturation.
dc.description.confidentialfalse
dc.format.paginatione0190839-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/29304106
dc.identifier.citationAnderson RC, MacGibbon AKH, Haggarty N, Armstrong KM, Roy NC. (2018). Bovine dairy complex lipids improve in vitro measures of small intestinal epithelial barrier integrity.. PLoS One. 13. 1. (pp. e0190839-).
dc.identifier.doi10.1371/journal.pone.0190839
dc.identifier.eissn1932-6203
dc.identifier.elements-typejournal-article
dc.identifier.issn1932-6203
dc.identifier.numberARTN e0190839
dc.identifier.piiPONE-D-17-35590
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71881
dc.languageeng
dc.publisherPLOS
dc.publisher.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0190839
dc.relation.isPartOfPLoS One
dc.rights(c) 2018 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectBiological Transport
dc.subjectCaco-2 Cells
dc.subjectCattle
dc.subjectDairying
dc.subjectHumans
dc.subjectIntestinal Mucosa
dc.subjectIntestine, Small
dc.subjectLipids
dc.subjectPermeability
dc.subjectTumor Necrosis Factor-alpha
dc.titleBovine dairy complex lipids improve in vitro measures of small intestinal epithelial barrier integrity
dc.typeJournal article
pubs.elements-id434477
pubs.organisational-groupOther
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