New targets acquired: Improving locus recovery from the Angiosperms353 probe set

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dc.citation.issue7
dc.citation.volume9
dc.contributor.authorMcLay T
dc.contributor.authorBirch J
dc.contributor.authorGunn B
dc.contributor.authorNing W
dc.contributor.authorTate J
dc.contributor.authorNauheimer L
dc.contributor.authorJoyce E
dc.contributor.authorSimpson L
dc.contributor.authorSchmidt-Lebuhn A
dc.contributor.authorBaker W
dc.contributor.authorForest F
dc.contributor.authorJackson C
dc.date.available2021-07
dc.date.available2021-03-15
dc.date.issued14/06/2021
dc.descriptionPublished source must be acknowledged
dc.description.abstractPREMISE: Universal target enrichment kits maximize utility across wide evolutionary breadth while minimizing the number of baits required to create a cost-efficient kit. The Angiosperms353 kit has been successfully used to capture loci throughout the angiosperms, but the default target reference file includes sequence information from only 6–18 taxa per locus. Consequently, reads sequenced from on-target DNA molecules may fail to map to references, resulting in fewer on-target reads for assembly, and reducing locus recovery. METHODS: We expanded the Angiosperms353 target file, incorporating sequences from 566 transcriptomes to produce a ‘mega353’ target file, with each locus represented by 17–373 taxa. This mega353 file is a drop-in replacement for the original Angiosperms353 file in HybPiper analyses. We provide tools to subsample the file based on user-selected taxon groups, and to incorporate other transcriptome or protein-coding gene data sets. RESULTS: Compared to the default Angiosperms353 file, the mega353 file increased the percentage of on-target reads by an average of 32%, increased locus recovery at 75% length by 49%, and increased the total length of the concatenated loci by 29%. DISCUSSION: Increasing the phylogenetic density of the target reference file results in improved recovery of target capture loci. The mega353 file and associated scripts are available at: https://github.com/chrisjackson-pellicle/NewTargets.
dc.description.publication-statusPublished
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000661202300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN e11420
dc.identifier.citationAPPLICATIONS IN PLANT SCIENCES, 2021, 9 (7)
dc.identifier.doi10.1002/aps3.11420
dc.identifier.elements-id446127
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2168-0450
dc.identifier.urihttps://hdl.handle.net/10179/16417
dc.publisherBotanical Society of America
dc.relation.isPartOfAPPLICATIONS IN PLANT SCIENCES
dc.subjectAngiosperms353
dc.subjectHybPiper
dc.subjectlocus recovery
dc.subjecttarget capture
dc.subjecttarget file
dc.subject.anzsrc0703 Crop and Pasture Production
dc.titleNew targets acquired: Improving locus recovery from the Angiosperms353 probe set
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Natural Sciences
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