Rapid yeast-based screen for Functionally Relevant Amino Acids (RS-FRAA) in a protein

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dc.citation.issue1
dc.citation.volume4
dc.contributor.authorGhuge AA
dc.contributor.authorAnderson RA
dc.contributor.authorGottfried S
dc.contributor.authorDaube C
dc.contributor.authorKoloamatangi SMBMJ
dc.contributor.authorSchiemann AH
dc.contributor.authorSattlegger E
dc.coverage.spatialUnited States
dc.date.accessioned2024-01-10T01:58:17Z
dc.date.accessioned2024-07-25T06:49:06Z
dc.date.available2023-01-25
dc.date.available2024-01-10T01:58:17Z
dc.date.available2024-07-25T06:49:06Z
dc.date.issued2023-03-17
dc.description.abstractHere, we describe a fast and cost-effective procedure to generate a large array of mutant proteins and immediately screen for those with altered protein function. This protocol is a modification from three existing approaches: fusion PCR, Saccharomyces cerevisiae in-yeast recombination, and semi-quantitative growth assays. We also describe a mating step to reduce the occurrence of false positive findings due to ectopic mutations. The only requirement is that the protein elicits a phenotype in Saccharomyces cerevisiae.
dc.description.confidentialfalse
dc.format.pagination101545-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36856772
dc.identifier.citationGhuge AA, Anderson RA, Gottfried S, Daube C, Koloamatangi SMBMJ, Schiemann AH, Sattlegger E. (2023). Rapid yeast-based screen for Functionally Relevant Amino Acids (RS-FRAA) in a protein.. STAR Protoc. 4. 1. (pp. 101545-).
dc.identifier.doi10.1016/j.xpro.2022.101545
dc.identifier.eissn2666-1667
dc.identifier.elements-typejournal-article
dc.identifier.issn2666-1667
dc.identifier.number101545
dc.identifier.piiS2666-1667(22)00425-7
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70946
dc.languageeng
dc.publisherElsevier Inc
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S2666166722004257
dc.relation.isPartOfSTAR Protoc
dc.rights(c) 2022 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBiotechnology and bioengineering
dc.subjectCell-based Assays
dc.subjectModel Organisms
dc.subjectMolecular Biology
dc.subjectSaccharomyces cerevisiae
dc.subjectAmino Acids
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectPhenotype
dc.titleRapid yeast-based screen for Functionally Relevant Amino Acids (RS-FRAA) in a protein
dc.typeJournal article
pubs.elements-id459407
pubs.organisational-groupOther
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