Methylome changes in Lolium perenne associated with long-term colonisation by the endophytic fungus Epichloë sp. LpTG-3 strain AR37.

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dc.citation.volume14
dc.contributor.authorForte FP
dc.contributor.authorMalinowska M
dc.contributor.authorNagy I
dc.contributor.authorSchmid J
dc.contributor.authorDijkwel P
dc.contributor.authorHume DE
dc.contributor.authorJohnson RD
dc.contributor.authorSimpson WR
dc.contributor.authorAsp T
dc.contributor.editorMorillas JIV
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-08-19T21:38:57Z
dc.date.available2024-08-19T21:38:57Z
dc.date.issued2023-11-13
dc.description.abstractEpichloë spp. often form mutualistic interactions with cool-season grasses, such as Lolium perenne. However, the molecular mechanisms underlying this interaction remain poorly understood. In this study, we employed reduced representation bisulfite sequencing method (epiGBS) to investigate the impact of the Epichloë sp. LpTG-3 strain AR37 on the methylome of L. perenne across multiple grass generations and under drought stress conditions. Our results showed that the presence of the endophyte leads to a decrease in DNA methylation across genomic features, with differentially methylated regions primarily located in intergenic regions and CHH contexts. The presence of the endophyte was consistently associated with hypomethylation in plants across generations. This research sheds new light on the molecular mechanisms governing the mutualistic interaction between Epichloë sp. LpTG-3 strain AR37 and L. perenne. It underscores the role of methylation changes associated with endophyte infection and suggests that the observed global DNA hypomethylation in L. perenne may be influenced by factors such as the duration of the endophyte-plant association and the accumulation of genetic and epigenetic changes over time.
dc.description.confidentialfalse
dc.edition.editionNovember 2023
dc.format.pagination1258100-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37810388
dc.identifier.citationForte FP, Malinowska M, Nagy I, Schmid J, Dijkwel P, Hume DE, Johnson RD, Simpson WR, Asp T. (2023). Methylome changes in Lolium perenne associated with long-term colonisation by the endophytic fungus Epichloë sp. LpTG-3 strain AR37.. Front Plant Sci. 14. (pp. 1258100-).
dc.identifier.doi10.3389/fpls.2023.1258100
dc.identifier.eissn1664-462X
dc.identifier.elements-typejournal-article
dc.identifier.issn1664-462X
dc.identifier.number125800
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71342
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1258100/full
dc.relation.isPartOfFront Plant Sci
dc.subjectDNA methylation
dc.subjectEpichloë sp.
dc.subjectLolium perenne
dc.subjectartificial association
dc.subjectdrought stress
dc.subjectendophytic fungi
dc.subjectgeneration effect
dc.subjectplant-microbe interactions
dc.titleMethylome changes in Lolium perenne associated with long-term colonisation by the endophytic fungus Epichloë sp. LpTG-3 strain AR37.
dc.typeJournal article
pubs.elements-id481010
pubs.organisational-groupOther
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