Development and validation of an LC-MS/MS method for the quantification of oral-sugar probes in plasma to test small intestinal permeability and absorptive capacity in the domestic cat (Felis catus)

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dc.citation.volume1242
dc.contributor.authorPatterson K
dc.contributor.authorFraser K
dc.contributor.authorBernstein D
dc.contributor.authorBermingham EN
dc.contributor.authorWeidgraaf K
dc.contributor.authorKate Shoveller A
dc.contributor.authorThomas D
dc.coverage.spatialNetherlands
dc.date.accessioned2024-10-09T22:17:17Z
dc.date.available2024-10-09T22:17:17Z
dc.date.issued2024-07-15
dc.description.abstractA novel method for quantifying the concentration of lactulose, rhamnose, xylose, and 3-O-methylglucose (3-OMG) in cat plasma using liquid chromatography-mass spectrometry (LC-MS) was developed. Domestic male cats (n = 13) were orally dosed with a solution containing the four sugars to test the permeability and absorptive capacity of their intestinal barrier. Plasma samples were taken 3 h later and were prepared with acetonitrile (ACN), dried under N2, and reconstituted in 90 % ACN with 1 mM ammonium formate. Stable isotope labelled 13C standards for each analyte were used as internal standards. Chromatographic separation was conducted using a Phenomenex Luna NH2 column with a gradient elution system of deionized water and 90 % ACN with 1 mM ammonium formate at 300 µL/min for 13 min total analysis time. Recovery trials were conducted in triplicate over three days with RSD values (%) for each day ranging from 1.2 to 1.4 for lactulose, 5.4 - 6.0 for rhamnose, 3.3 - 5.5 for xylose, and 2.6 - 5.6 for 3-OMG. Inter-day variations for each analyte were not different (p > 0.05). Limit of detection and quantification were 0.2 and 0.7 µg/mL for lactulose, 0.8 and 2.4 µg/mL for rhamnose, 0.6 and 1.8 µg/mL for xylose, and 0.3 and 1.1 µg/mL for 3-OMG, respectively. Plasma sugar concentrations recovered from cats were above the limit of quantification and below the highest calibration standard, validating the use of this method to test intestinal permeability and absorptive capacity in cats.
dc.description.confidentialfalse
dc.format.pagination124216-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/38917651
dc.identifier.citationPatterson K, Fraser K, Bernstein D, Bermingham EN, Weidgraaf K, Kate Shoveller A, Thomas D. (2024). Development and validation of an LC-MS/MS method for the quantification of oral-sugar probes in plasma to test small intestinal permeability and absorptive capacity in the domestic cat (Felis catus).. J Chromatogr B Analyt Technol Biomed Life Sci. 1242. (pp. 124216-).
dc.identifier.doi10.1016/j.jchromb.2024.124216
dc.identifier.eissn1873-376X
dc.identifier.elements-typejournal-article
dc.identifier.issn1570-0232
dc.identifier.number124216
dc.identifier.piiS1570-0232(24)00225-3
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71663
dc.languageeng
dc.publisherElsevier BV
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S1570023224002253
dc.relation.isPartOfJ Chromatogr B Analyt Technol Biomed Life Sci
dc.rights(c) The author/sen
dc.rights.licenseCC BYen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAbsorptive capacity
dc.subjectDifferential sugar absorption test
dc.subjectIntestinal permeability
dc.subjectLC-MS/MS
dc.subjectPlasma
dc.subjectTandem mass spectrometry
dc.subjectAnimals
dc.subjectCats
dc.subjectTandem Mass Spectrometry
dc.subjectMale
dc.subjectReproducibility of Results
dc.subjectChromatography, Liquid
dc.subjectLinear Models
dc.subjectIntestine, Small
dc.subjectPermeability
dc.subjectIntestinal Absorption
dc.subjectLimit of Detection
dc.subjectAdministration, Oral
dc.subjectLiquid Chromatography-Mass Spectrometry
dc.subjectIntestinal Barrier Function
dc.titleDevelopment and validation of an LC-MS/MS method for the quantification of oral-sugar probes in plasma to test small intestinal permeability and absorptive capacity in the domestic cat (Felis catus)
dc.typeJournal article
pubs.elements-id489449
pubs.organisational-groupOther
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