Browsing by Author "Millner JP"

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    Assessment of manuka provenances for production of high ‘unique manuka factor’ honey
    (Agronomy Society of New Zealand, 2013) Millner JP; Hamilton G; Robertson A; Stephens J; Hill, GD; McGill, CR
    Manuka (Leptospermum scoparium) honey with high methylglyoxal content, commonly expressed as unique manuka factor (UMF®) content, has strong antibacterial and anti-fungal properties. Consequently, there is a strong demand for high UMF manuka honey in the health food industry both in New Zealand and overseas. Currently manuka honey is produced from natural stands of manuka, but UMF content varies among regions. The active ingredient methylglyoxal is produced by natural chemical transformation of dihydroxyacetone (DHA) present in the nectar. Production of high UMF honey is insufficient to meet market demand; this is due to variation in UMF amongst stands causing unpredictability in quality as well as inaccessibility of many manuka stands. The feasibility of increasing production by establishing manuka plantations using plants known to produce nectar with high DHA content is being investigated. This study compared establishment, growth and nectar DHA content of four manuka provenances; two from Northland, and one each from Waikato and Wairarapa in a replicated, randomised complete block trial (Site A). In addition a manuka plantation of a single provenance was monitored for nectar DHA content (Site B). Both sites are in the Whanganui area. Survival of seedlings in the Site A trial at 12 months was high in all provenances. Nectar DHA content ranged from 3666 to 6902 mg/kg 80° Brix and there were no significant differences amongst the provenances. These DHA levels were considerably higher than levels measured in the local manuka (2565 mg/kg 80° Brix). At Site B nectar DHA content of the plantation manuka (5770 mg/kg 80° Brix) was significantly (P=0.05) higher than the indigenous manuka (2565 mg/kg 80° Brix). Early results suggest that manuka provenances can be utilised on different sites to produce high DHA nectar and ultimately high UMF manuka honey, providing landowners with an additional income and help prevent erosion of marginal hill country.
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    Bioeconomic Modelling to Assess the Impacts of Using Native Shrubs on the Marginal Portions of the Sheep and Beef Hill Country Farms in New Zealand (article)
    (MDPI (Basel, Switzerland), 2021-10-18) Wangui JC; Kenyon PR; Tozer PR; Millner JP; Pain SJ
    New Zealand hill country sheep and beef farms contain land of various slope classes. The steepest slopes have the lowest pasture productivity and livestock carrying capacity and are the most vulnerable to soil mass movements. A potential management option for these areas of a farm is the planting of native shrubs which are browsable and provide erosion control, biodiversity, and a source of carbon credits. A bioeconomic whole farm model was developed by adding a native shrub sub-model to an existing hill country sheep and beef enterprise model to assess the impacts on feed supply, flock dynamics, and farm economics of converting 10% (56.4 hectares) of the entire farm, focusing on the steep slope areas, to native shrubs over a 50-year period. Two native shrub planting rates of 10% and 20% per year of the allocated area were compared to the status quo of no (0%) native shrub plantings. Mean annual feed supply dropped by 6.6% and 7.1% causing a reduction in flock size by 10.9% and 11.6% for the 10% and 20% planting rates, respectively, relative to 0% native shrub over the 50 years. Native shrub expenses exceeded carbon income for both planting rates and, together with reduced income from sheep flock, resulted in lower mean annual discounted total sheep enterprise cash operating surplus for the 10% (New Zealand Dollar (NZD) 20,522) and 20% (NZD 19,532) planting scenarios compared to 0% native shrubs (NZD 22,270). All planting scenarios had positive Net Present Value (NPV) and was highest for the 0% native shrubs compared to planting rates. Break-even carbon price was higher than the modelled carbon price (NZD 32/ New Zealand Emission Unit (NZU)) for both planting rates. Combined, this data indicates planting native shrubs on 10% of the farm at the modelled planting rates and carbon price would result in a reduction in farm sheep enterprise income. It can be concluded from the study that a higher carbon price above the break-even can make native shrubs attractive in the farming system.
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    Development of Plant-Fungal Endophyte Associations to Suppress Phoma Stem Canker in Brassica
    (MDPI (Basel, Switzerland), 2021-11-19) Roodi D; Millner JP; McGill CR; Johnson RD; Hea S-Y; Brookes JJ; Glare TR; Card SD; Santoyo G; White J; Kumar A; Mishra V
    Endophytic microorganisms are found within the tissues of many plants species, with some conferring several benefits to the host plant including resistance to plant diseases. In this study, two putative endophytic fungi that were previously isolated from wild seeds of Brassica, identified as Beauveria bassiana and Pseudogymnoascus pannorum, were inoculated into cultivars of three Brassica species-Brassica napus, Br. rapa and Br. oleracea. Both fungal endophytes were reisolated from above- and below-ground tissues of inoculated plants at four different plant-growth stages, including cotyledon, one-leaf, two-leaf, and four-leaf stages. None of the plants colonised by these fungi exhibited any obvious disease symptoms, indicating the formation of novel mutualistic associations. These novel plant-endophyte associations formed between Brassica plants and Be. bassiana significantly inhibited phoma stem canker, a devastating disease of Brassica crops worldwide, caused by the fungal pathogen Leptosphaeria maculans. The novel association formed with P. pannorum significantly suppressed the amount of disease caused by L. maculans in one out of two experiments. Although biological control is not a new strategy, endophytic fungi with both antiinsect and antifungal activity are a highly conceivable, sustainable option to manage pests and diseases of economically important crops.
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    Effects of Sheep Grazing Systems on Water Quality with a Focus on Nitrate Leaching
    (MDPI (Basel, Switzerland), 2022-06-01) Maheswaran S; Cranston LM; Millner JP; Horne DJ; Hanly JA; Kenyon PR; Kemp PD
    This article reviews the literature on nitrate leaching under sheep grazing systems and focuses on identifying future research needs. Urinary nitrogen (N) is an important source of the nitrate leached from pastoral agriculture. Urinary N excretion can be measured or simulated using models and has been well characterised for dairy systems. It is difficult to continuously monitor the urinary N excretion of sheep under field conditions; consequently, measurements of N excretion in sheep urine are limited. Urination events by sheep vary greatly in volume (0.5 L to 6.9 L), concentration (3 to 13.7 g N/L), and frequency (8 to 23 events/day); this variation results in a corresponding variation in N loading rates in urine patches. The amount of nitrate leached under pastures grazed by sheep has typically varied between 1 and 50 kg N/ha/year, but rates as high as 300 kg N/ha/year have been reported. The quantity of nitrate leached under sheep depends on the season, climate, quantity and timing of drainage, the interaction between forage production and stocking rate, fertiliser applied, N fixation by legumes, forage type, and grazing management. The majority of studies examining nitrate leaching under sheep grazing systems are more than 20 years old; so, there is little recent information on nitrate leaching under modern pasture-based sheep production systems. Further research is required to quantify nitrate leaching levels under current sheep farming practices, to understand the impacts of this leaching on water quality, and to help identify effective strategies to reduce the transfer of N from grazed paddocks to receiving water bodies. This additional information will help provide information for decision support tools, including models and management practices, to help sheep farmers minimise their impact on the aquatic environment.
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    Evaluation of sorghum, sudan-grass and pearl millet cultivars in Manawatu
    (Agronomy Society of New Zealand, 2013-09) Millner JP; Silungwe D; McGill CR; McGill, CR; Hill, GD
    Sorghum, sudan-grass and pearl millet are versatile summer forages able to be grazed or conserved as silage. However, there is little recently published information on the performance these crops in New Zealand. A trial was carried out at Massey University, Palmerston North, to compare forage yields and crop morphology of four sorghum x sudan-grass hybrids (Pac 8421, Pac 8423, Pacific BMR and Bettagraze), two sudan-grass (Superdan 2 and Sprint) and one pearl millet (Nutrifeed) cultivars sown on the 8 and 21 December 2009. At final harvest, 57 and 58 days after sowing for the first and second sowing dates respectively, dry matter yields decreased (P<0.005) with delayed sowing from 12,792 kg ha-1 to 11,356 kg ha-1. There were cultivar differences (P<0.0001) at both sowing dates. Mean yields (across sowing dates) ranged from 9,823 (Nutrifeed) to 13,953 kg DM ha-1 (Pac 8423). There was a significant interaction (P<0.0001) between sowing date and cultivar for yield at the second harvest; cultivar differences for the first sowing date were larger than for the second, which suffered more from cool autumn temperatures. There was a strong, positive, linear relationship between plant height at harvest and DM yield (R2 = 0.69). Mean leaf:stem ratio, for the first sowing (1.9), was significantly (P<0.001) higher than in the second sowing (1.8) and was influenced by cultivar.
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    Grazing activity increases decomposition of yak dung and litter in an alpine meadow on the Qinghai-Tibet plateau
    (Springer Nature Switzerland AG on behalf of the Royal Netherlands Society of Agricultural Science, 2019-11) Yang C; Zhang Y; Hou F; Millner JP; Wang Z; Chang S; Shang Z
    Aims: This study investigated the influences of herbivore grazing intensity and grazing season on decomposition and nutrient release of dung and litter, which aimed to improve our understandings of grazing affecting nutrient cycling in alpine meadows on the Qinghai-Tibetan Platean. Methods: A factorial design experiment comprising 3 grazing intensities (non-grazing, moderate grazing, and heavy grazing) and 2 grazing seasons (summer and winter), was applied to quantify the decomposition and chemistry of dung and litter in an alpine pasture using the litterbag technique. Litterbags were retrieved for analysis of mass loss and nutrient release with 180, 360, 540, and 720 days after placement. Results: Grazing activity accelerated the decomposition of dung and litter and increased nutrient release from dung and litter by increasing soil temperature compared with non-grazing pastures, whereas grazing season had no effect on decomposition. The decomposition time was shorter for dung than that for litter. Conclusions: Herbivores grazing benefited dung and litter decomposition and nutrient cycling directly by increasing soil temperature, which is likely to promote soil microbial activity due to low temperatures in alpine meadows, and indirectly through herbage ingestion and dung deposition which increase the organic debris concentration used for microorganisms growth and reproduction. This study provides insights into the mechanisms of grazing regulating nutrient cycling in alpine ecosystems.
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    In Vitro Fermentation of Browsable Native Shrubs in New Zealand
    (MDPI (Basel, Switzerland), 2022-08-10) Wangui JC; Millner JP; Kenyon PR; Tozer PR; Morel PCH; Pain SJ
    Information on the nutritive value and in vitro fermentation characteristics of native shrubs in New Zealand is scant. This is despite their potential as alternatives to exotic trees and shrubs for supplementary fodder, and their mitigation of greenhouse gases and soil erosion on hill-country sheep and beef farms. The objectives of this study were to measure the in vitro fermentation gas production, predict the parameters of the in vitro fermentation kinetics and estimate the in vitro fermentation of volatile fatty acids (VFA), microbial biomass (MBM), and greenhouse gases of four native shrubs ( Coprosma robusta, Griselinia littoralis, Hoheria populnea, and Pittosporum crassifolium) and an exotic fodder tree species, Salix schwerinii. The total in vitro gas production was higher (p < 0.05) for the natives than for the S. schwerinii. A prediction using the single-pool model resulted in biologically incorrect negative in vitro total gas production from the immediately soluble fraction of the native shrubs. However, the dual pool model better predicted the in vitro total gas production and was in alignment with the measured in vitro fermentation end products. The in vitro VFA and greenhouse gas production from the fermentation of leaf and stem material was higher (p < 0.05), and the MBM lower (p < 0.05), for the native shrubs compared to the S. schwerinii. The lower in vitro total gas production, VFA, and greenhouse gases production and higher MBM of the S. schwerinii may be explained by the presence of condensed tannins (CT), although this was not measured and requires further study. In conclusion, the results from this study suggest that when consumed by ruminant livestock, browsable native shrubs can provide adequate energy and microbial protein, and that greenhouse-gas production from these species is within the ranges reported for typical New Zealand pastures.
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    Methylobacterium, a major component of the culturable bacterial endophyte community of wild Brassica seed
    (PeerJ, Inc, 2020-07-10) Roodi D; Millner JP; McGill C; Johnson RD; Jauregui R; Card SD; Souza V
    BACKGROUND: Plants are commonly colonized by a wide diversity of microbial species and the relationships created can range from mutualistic through to parasitic. Microorganisms that typically form symptomless associations with internal plant tissues are termed endophytes. Endophytes associate with most plant species found in natural and managed ecosystems. They are extremely important plant partners that provide improved stress tolerance to the host compared with plants that lack this symbiosis. Plant domestication has reduced endophyte diversity and therefore the wild relatives of many crop species remain untapped reservoirs of beneficial microbes. Brassica species display immense diversity and consequently provide the greatest assortment of products used by humans from a single plant genus important for agriculture, horticulture, bioremediation, medicine, soil conditioners, composting crops, and in the production of edible and industrial oils. Many endophytes are horizontally transmitted, but some can colonize the plant's reproductive tissues, and this gives these symbionts an efficient mechanism of propagation via plant seed (termed vertical transmission). METHODS: This study surveyed 83 wild and landrace Brassica accessions composed of 14 different species with a worldwide distribution for seed-originating bacterial endophytes. Seed was stringently disinfected, sown within sterile tissue culture pots within a sterile environment and incubated. After approximately 1-month, direct isolation techniques were used to recover bacterial endophytes from roots and shoots of symptomless plants. Bacteria were identified based on the PCR amplification of partial 16S rDNA gene sequences and annotated using the BLASTn program against the NCBI rRNA database. A diversity index was used as a quantitative measure to reflect how many different bacterial species there were in the seed-originating microbial community of the Brassica accessions sampled. RESULTS: Bacterial endophytes were recovered from the majority of the Brassica accessions screened. 16S rDNA gene sequencing identified 19 different bacterial species belonging to three phyla, namely Actinobacteria, Firmicutes and Proteobacteria with the most frequently isolated species being Methylobacterium fujisawaense, Stenotrophomonas rhizophila and Pseudomonas lactis. Methylobacterium was the dominant genus composing 56% of the culturable isolated bacterial community and was common in 77% of accessions possessing culturable bacterial endophytes. Two selected isolates of Methylobacterium significantly promoted plant growth when inoculated into a cultivar of oilseed rape and inhibited the growth of the pathogen Leptosphaeria maculans in dual culture. This is the first report that investigates the seed-originating endophytic microorganisms of wild Brassica species and highlights the Brassica microbiome as a resource for plant growth promoting bacteria and biological control agents.
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    The dominance of Ligularia spp. related to significant changes in soil microenvironment
    (Elsevier B.V., 2021-09-09) Ade L; Millner JP; Hou F
    Exploring how plants adapt to and change the surrounding environment has become essential to understanding their survival strategies and co-evolution mechanisms. Ligularia virgaurea and Ligularia sagitta are the two most common species in the alpine grazing ecosystems of the eastern Qinghai-Tibetan Plateau (QTP) and becoming increasingly dominant. Studies have suggested that overgrazing has allowed Ligularia to gain a competitive advantage by changing plant community structure, which is often closely related to the soil environment. However, we don't fully understand the soil environment changes during this process, and the underlying mechanisms have not been explored. Therefore, we investigated plant community characteristics, soil fertility and soil microbial diversity in the L. virgaurea and L. sagitta communities on the eastern QTP. Ligularia spp. significantly changed the plant community by reducing biomass, vegetation coverage, abundance, and biodiversity, and the effect of L. sagitta on the plant community was stronger than that of L. virgaurea. In the plant communities dominated by L. virgaurea and L. sagitta, soil nutrients and soil microbial communities changed significantly. Aggregated boosted trees analysis revealed that soil Mg levels had the greatest relative influence on the structure and diversity of the soil microbial community. Our study provides data and a theoretical basis for revealing the survival strategies of L. sagitta and L. virgaurea and, provides a basis for weed management in grazed ecosystems.
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