Massey Research Online - Browsing by Author "Ghanizadeh H"

Browsing by Author "Ghanizadeh H"

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A novel mutation in IAA16 is associated with dicamba resistance in Chenopodium album
(John Wiley and Sons Ltd on behalf of Society of Chemical Industry, 2024-07) Ghanizadeh H; He L; Griffiths AG; Harrington KC; Carbone V; Wu H; Tian K; Bo H; Xinhui D
BACKGROUND: Resistance to dicamba in Chenopodium album was first documented over a decade ago, however, the molecular basis of dicamba resistance in this species has not been elucidated. In this research, the resistance mechanism in a dicamba-resistant C. album phenotype was investigated using a transcriptomics (RNA-sequence) approach. RESULTS: The dose-response assay showed that the resistant (R) phenotype was nearly 25-fold more resistant to dicamba than a susceptible (S) phenotype of C. album. Also, dicamba treatment significantly induced transcription of the known auxin-responsive genes, Gretchen Hagen 3 (GH3), small auxin-up RNAs (SAURs), and 1-aminocyclopropane-1-carboxylate synthase (ACS) genes in the susceptible phenotype. Comparing the transcripts of auxin TIR/AFB receptors and auxin/indole-3-acetic acid (AUX/IAA) proteins identified from C. album transcriptomic analysis revealed that the R phenotype contained a novel mutation at the first codon of the GWPPV degron motif of IAA16, resulting in an amino acid substitution of glycine (G) with aspartic acid (D). Sequencing the IAA16 gene in other R and S individuals further confirmed that all the R individuals contained the mutation. CONCLUSION: In this research, we describe the dicamba resistance mechanism in the only case of dicamba-resistant C. album reported to date. Prior work has shown that the dicamba resistance allele confers significant growth defects to the R phenotype investigated here, suggesting that dicamba-resistant C. album carrying this novel mutation in the IAA16 gene may not persist at high frequencies upon removal of dicamba application.
A Study of the Interaction, Morphology, and Structure in Trypsin-Epigallocatechin-3-Gallate Complexes
(MDPI (Basel, Switzerland), 2021-07-28) Liu J; Ghanizadeh H; Li X; Han Z; Qiu Y; Zhang Y; Chen X; Wang A; Tresserra-Rimbau A; Bresciani L
Understanding the interaction between proteins and polyphenols is of significance to food industries. The aim of this research was to investigate the mode of aggregation for trypsin-EGCG (Epigallocatechin-3-gallate) complexes. For this, the complex was characterized by fluorescence spectroscopy, circular dichroism (CD) spectra, small-angel X-ray scattering (SAXS), and atomic force microscope (AFM) techniques. The results showed that the fluorescence intensity of trypsin-EGCG complexes decreased with increasing the concentration of EGCG, indicating that the interaction between trypsin and EGCG resulted in changes in the microenvironment around fluorescent amino acid residues. The results of CD analysis showed conformational changes in trypsin after binding with EGCG. The results from SAXS analysis showed that the addition of EGCG results in the formation of aggregates of trypsin-EGCG complexes, and increasing the concentration of EGCG resulted in larger aggregates. AFM images showed that the trypsin-EGCG complex formed aggregates of irregular ellipsoidal shapes with the size of about 200 × 400 × 200 nm, with EGCG interconnecting the trypsin particles. Overall, according to these results, it was concluded that the large aggregates of trypsin-EGCG complexes are formed from several small aggregates that are interconnected. The results of this study shed some light on the interaction between digestive enzymes and EGCG.
Clonostachys rosea Promotes Root Growth in Tomato by Secreting Auxin Produced through the Tryptamine Pathway
(MDPI (Basel, Switzerland), 2022-11-04) Han Z; Ghanizadeh H; Zhang H; Li X; Li T; Wang Q; Liu J; Wang A; Feng M-G
Clonostachys rosea (Link) Schroers is a filamentous fungus that has been widely used for biological control, biological fermentation, biodegradation and bioenergy. In this research, we investigated the impact of this fungus on root growth in tomato and the underlying mechanisms. The results showed that C. rosea can promote root growth in tomato, and tryptophan enhances its growth-promoting impacts. The results also showed that tryptophan increases the abundance of metabolites in C. rosea, with auxin (IAA) and auxin-related metabolites representing a majority of the highly abundant metabolites in the presence of tryptophan. It was noted that C. rosea could metabolize tryptophan into tryptamine (TRA) and indole-3-acetaldehyde (IAAId), and these two compounds are used by C. rosea to produce IAA through the tryptamine (TAM) pathway, which is one of the major pathways in tryptophan-dependent IAA biosynthesis. The IAA produced is used by C. rosea to promote root growth in tomato. To the best of our knowledge, this is the first report on IAA biosynthesis by C. rosea through the TAM pathway. More research is needed to understand the molecular mechanisms underlying IAA biosynthesis in C. rosea, as well as to examine the ability of this fungus to boost plant development in the field.
Efficacy of herbicides for selective control of an invasive liana, old man's beard (Clematis vitalba)
(Published by Cambridge University Press on behalf of the Weed Science Society of America, 2023-06-02) Jarvis-Lowry B; Harrington KC; Ghanizadeh H; Robertson AW
Old man's beard is a woody liana that has become an invasive weed in many areas of its introduction, through its vigorous spread and negative impacts on the tree hosts it climbs. Control techniques that improve precision and reduce non-target damage are increasingly preferred for weed control yet have not been compared in published research for use against old man's beard. Field experiments in New Zealand were conducted to: (i) assess targeted herbicide techniques for control of this weed's climbing stems when growing among trees and (ii) assess foliar herbicides for control of creeping stems in ruderal sites. For climbing stems, triclopyr in oil was applied around the circumference of woody stems near their base, which was compared with cutting the stems and applying concentrated glyphosate gel (45% ai) to each cut end. Herbicides were applied in autumn directly to individual stem bases of the weed, thereby protecting tree hosts and other non-target vegetation. The basal application of triclopyr to intact stems was highly effective (>95% mortality) with no damage to nearby trees noted. The glyphosate gel applications to cut stems were less effective (56% mortality by 2 yr after treatment). For creeping stems in grass-dominated ruderal sites, selective foliar herbicide sprays had not been previously juxtaposed to compare control of old man's beard. Three selective sprays that do not damage existing grass cover were applied in autumn at their recommended rates: (i) metsulfuron; (ii) triclopyr; and (iii) a mixture of triclopyr, picloram, and aminopyralid. All herbicide treatments provided effective control, although metsulfuron had a negative effect on grass vigor, which might allow new establishment of old man's beard seedlings by competitive release. These results provide effective options that reduce non-target damage for control of both climbing and creeping old man's beard stems.
Natural Variation in Chromium Accumulation and the Development of Related EST-SSR Molecular Markers in Miscanthus sinensis
(MDPI (Basel, Switzerland), 2024-07-05) Nie G; Liu A; Ghanizadeh H; Wang Y; Tang M; He J; Feng G; Huang L; Zhang X; Tani E
Soil pollution by heavy metals is a serious environmental concern globally. Hexavalent (VI) chromium (Cr) is one of the main pollutants causing groundwater and soil heavy metal pollution. Miscanthus sinensis is a C4 perennial grass species with a high level of heavy metal tolerance. This species can effectively remove Cr from soils and maintain desirable biomass production under Cr stress. This research aimed to characterize and compare Cr accumulation in 58 genotypes of M. sinensis and to develop Expressed Sequence Tag–Simple Sequence Repeat (EST-SSR) markers associated with Cr tolerance. The results show that the pattern of translocation of Cr in plants differed among the 58 M. sinensis genotypes following treatment of 200 mg/L of Cr6+; however, in most genotypes, the Cr was primarily accumulated in roots. A total of 43,367 EST-SSRs were identified, and 88 EST-SSR primer pairs corresponding to candidate genes involved in Cr accumulation in M. sinensis were selected for validation. Subsequently, 170 polymorphic loci generated from 24 validated EST-SSRs were used for the population structure and marker–trait association analysis. Based on a general linear model (GLM), a total of 46 associations were identified (p < 0.05), with 14 EST-SSRs markers associated with target traits. Among them, four genes related to ABC transporters, wall-associated receptor kinases, as well as two high-affinity sulfate transporters (ST), were identified under Cr stress (p < 0.05). The results of this study help to accelerate the screening across M. sinensis genotypes for desirable traits under Cr stress and provide a platform for M. sinensis genetic improvement and molecular-marker-assisted breeding.
Potential importance of vegetative spread and fragment regeneration for invasiveness of Clematis vitalba
(John Wiley and Sons Ltd on behalf of European Weed Research Society, 2024-05-12) Jarvis-Lowry B; Harrington KC; Ghanizadeh H; Robertson AW; Novak S
Identifying characteristics of invasive species or growth forms that facilitate their range expansion is critical for management. Clematis vitalba L. (old man's beard) is an invasive temperate liana in many areas of its introduction, yet its seedlings do not thrive in circumstances where resources are limited. Although some lianas in both tropical and temperate climates have been shown to spread by clonal stems along the ground, the bulk of previous research on C. vitalba reproduction has focused largely on aspects of seed ecology. The vegetative growth of the species is poorly understood. The first objective of our study was to evaluate the use of vegetative spread by C. vitalba as a means of local dispersal and population growth. We excavated ten 1-m2 plots in infested riparian zones and found an extensive, branching network of creeping stems, both above and below ground. Our second objective was to test the ability of C. vitalba stem fragments to act as vegetative propagules. After 4 months, ~50% of two-node fragments had regenerated, from both creeping and climbing stems. These studies help explain how a temperate liana forms populations and dominate ecological communities. The findings provide good evidence that C. vitalba may rely quite heavily on asexual reproduction. In addition, the results document liana stem phenotypic plasticity; fragmented climbing stems are just as likely as fragmented creeping stems to reprogram shoot tissue systems, generate roots and regrow as independent plants.
Soil Microbial Community Composition and Diversity Are Insusceptible to Nitrogen Addition in a Semi-Arid Grassland in Northwestern China
(MDPI (Basel, Switzerland), 2023-10-11) Tuo H; Li M; Ghanizadeh H; Huang J; Yang M; Wang Z; Wang Y; Tian H; Ye F; Li W; Monokrousos N
Human-caused nitrogen (N) deposition is a global environmental issue that can change community composition, functions, and ecosystem services. N deposition affects plants, soil, and microorganisms regionally and is linked to ecosystem, soil, and climate factors. We examined the effects of six N addition levels (0, 2.34 g, 4.67, 9.34,18.68, and 37.35 g N m−2 yr−1) on aboveground vegetation, surface soil properties, and microbial community. Alterations in microbial communities in response to N addition were monitored using 16S rRNA (16S ribosomal ribonucleic acid, where S donates a sedimentation coefficient) and ITS (internal transcribed spacer) regions for bacterial and fungal communities, respectively. N addition positively affected aboveground vegetation traits, such as biomass and community weighted mean of leaf nitrogen. N addition also limited phosphorus (P) availability and altered the microbial community assembly process from random processes to deterministic processes. The microbial community diversity and composition, however, were not sensitive to N addition. Partial least squares structural equation models showed that the composition of bacterial communities was mainly driven by the composition of plant communities and total nitrogen, while the composition of fungal communities was driven by soil pH and community weighted mean of leaf nitrogen. Taken together, the results of this research improved our understanding of the response of grassland ecosystems to N deposition and provided a theoretical basis for grassland utilization and management under N deposition.
Tolerance of plantain (Plantago lanceolata) to translocated herbicides wiped on flower stems
(2024-07-08) Shrivastav N; Harrington KC; Kemp PD; He XZ; Ghanizadeh H
Viability and dormancy of the Clematis vitalba aerial seed bank.
(John Wiley & Sons, Inc on behalf of German Society for Plant Sciences, Royal Botanical Society of the Netherlands., 2024-04-01) Jarvis-Lowry B; Harrington KC; Ghanizadeh H; Robertson AW; Bentsink L
Old man's beard (Clematis vitalba L.) is a liana species that has become invasive in many areas of its introduced range. Seeds are produced in abundance and are both physiologically and morphologically dormant upon maturity. To understand the importance of seeds to its invasiveness, changes in viability and dormancy of the aerial seed bank were tracked throughout the after-ripening period and during storage. Seeds collected every second month for 2 years were subjected to germination tests. Other seeds stored in outdoor ambient conditions or in a dry, chilled state were dissected before, during, and after imbibition, as well as during incubation, to measure embryo size. Less than 72% of seeds on the mother plant were viable. Viable seeds remained completely morpho-physiologically dormant throughout autumn, even when treated with nitrate. Physiological dormancy declined in response to seasonal changes, yet morphological dormancy did not change until seeds had been exposed to appropriate germination conditions for several days. Fully dormant autumn seeds decayed at higher rates during incubation than partially or fully after-ripened seeds, which were also more germinable and less dormant. Furthermore, seeds incubated in complete darkness were more likely to decay or remain dormant than those exposed to light. This study demonstrates that fewer than three-quarters of seeds produced are viable and further decay occurs after dispersal, yet total fertility is still very high, with enormous propagule pressure from seeds alone. Viable seeds are protected with two forms of dormancy; morphological dormancy requires additional germination cues in order to break after seasonal changes break physiological dormancy.

Browsing by Author "Ghanizadeh H"

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